Expression And Significance Of CD44V6, VEGF And JNK In Gastric Cancer And The Lymphatic Metastatic Tumors

Purpose: Gastric cancer is the fourth most common cancer and the second leading cancer-related death world wide because of its rapid growth and strong tendency of local invasiveness and distal metastasis. Recent researches found that CD44V6 and VEGF-C may have a relationship with the oncogenesis, tumor prog- ression and metastasis of some carcinomas. Along with constant acquaintance of c-Jun and JNK family members, researches found that JNK play a important role in the oncogenesis, tumor progression of gastric cancer. Investigation on expression of JNK, CD44V6 and VEGF-C in gastric cancer may provede some clues in the oncogensis, progression and metastasis of gastric cancer and be helpful for its diagnosis﹑anti-tumor treatment and prognosis evaluation.Method: S-P immunhistochemical stain was employed to detect the expression of CD44V6, VEGF-C and JNK in 20 cases of normal gastric tissue, 40 cases of gastric cancers in which 20 lymphatic metastasis. The expression of CD44V6, VEGF-C and JNK was compared amony normal gastric tissue, primary gastric cancer and the lymphatic metastatic tumor. The difference of their expression among different groups of patient's gender, age, tumor size, stage and differentiation was also analyzed.Result:1. Approximately two third 62.5% of the gastric cancer cases are positive for CD44V6 Expression, with a degree of intensty from weak (+) to strong (+++) more than the positive cases in normal gastric tissue 0%, The difference between the two groups is significant (P<0.01). 80% of the lymphatic metastatic tumors are positive for CD44V6 expression, more than the positive cases in primary gastric cancer 62.5%, The difference between the two groups is highly significant (P<0.01).2. Approximately half 50% of the gastric cancer cases are positive for VEGF-C Expression, more than the positive cases in normal gastric tissue 5% in which the intensity is all weak (+), The difference between the two groups is significant (P<0.01). 70% of the lymphatic metastatic tumors are positive for VEGF-C expression, more than the positive cases in primary gastric cancer 50% and even more cases with a strong (+++) intensity. The difference between the two groups is highly significant (P<0.01).3. Approximately three third75% of the gastric cancer cases are positive for JNK Expression, more than the positive cases in benign pathology and normal gastric tissue 5%, The difference between the two groups is highly significant (P<0.05). 60% of the lymphatic metastatic tumors are positive for JNK expression, less than the positive cases in primary gastric cancer 75%, The difference between the two groups is insignificant (P>0.05).4. The expression intensity of CD44V6, VEGF-C and JNK between any pairs of them in gastric cancer and metastasis of gastric cancer are positively correlated. In the case of gastric cancer, Correlation Analysis between CD44V6 with VEGF-C concluded that rs=0.342 and be significant (P<0.05) in gastric cancer. And rs=0.894 and be highly significant (P<0.01) in metastasis of gastric cancer. Correlation Analysis between CD44V6 with JNK concluded that rs=0.335 and be significant (P<0.05) in gastric cancer. And rs=0.843 and be highly significant (P<0.01) in metastasis of gastric cancer.Correlation Analysis between VEGF with JNK concluded that rs=0.341 and be significant (P<0.05) in gastric cancer. And rs=0.942 and be highly significant (P<0.01) in metastasis of gastric cancer.5. JNK expression in primary gastric cancer between different groups of tumor size, stage differentiation are significant, but between different groups of patient's gender, age differentiation are all not significant. CD44V6 and VEGF-C expression in primary gastric cancer between different groups of stage differentiation are significant, but between different groups of patient's gender, age tumor sizes are all not significant. CD44V6,VEGF-C and JNK expression in gastricadenocarcinoma between different groups of differentiation are all not significant.Conclusion:1. The positive rates of CD44V6, VEGF-C and JNK expression in gastric cancer observed using immunohistochemical stain are higher than normal gastric cancer and expression intensity is stronger in many of the positive cases. The positive rates of CD44V6 and VEGF-C expression in lymphatic metastatic tumor are higher than the primary gastric cancer and intensity is stronger in most of the positive cases. The difference of the positive rates and the expression intensity of JNK between the lymphatic metastatic tumors with the primary gastric cancer is insignificant (P>0.05). The expression intensity of CD44V6, VEGF-C and JNK in primary gastric cancer and metastastic tumor is positively correlated. So JNK maybe play a important role in the oncogenesis, tumor progression and metastasis of gastric cancer. So we believe that JNK be concerned with tumor metastasis, but the mechanism is unknown yet.2.JNK expression in primary gastric cancer between different groups of tumor size, stage differentiation are significant, but between different groups of patient's gender, age differentiation are all not significant. CD44V6 and VEGF-C expression in primary gastric cancer between different groups of stage differentiation are significant, but between different groups of patient's gender, age tumor sizes are all not significant. CD44V6,VEGF-C and JNK expression in gastricadenocarcinoma between different groups of differentiation are all not significant.