Expression And Significance Of VEGF And Glut-1 In Human Pancreatic Carcinoma

Primary pancreatic carcinoma(PPC) as a common malignant tumor in alimentary is a clinical symptom to hide ,it make progress quickly, and its prognosis is very bad and the existence rate of PPC is very low.Its biology characteristics: easily invasion nerve and blood vessel, the rate of lymphometastasis is high, and the anatomy position of PPC is deep, and lack the particularity toumor marker and the clinical manifestations in early grade, etc.As the same time, the postoperative recurrence or metastasis of PPC is easy occurrence. 75% of the accurate clinical diagnosis of PPC or more is already belong to the medium or later period, the surgical operation excision rate of PPC is low, and its not sensitive to the chemotherapy and radiotherapy. The existence rate of 5 years is still 5%.After the comprehensive treatment , it is predicting the tumor to relapse and metastasis, evaluating the prognosis of PPC that is a key place to raise the existence rate.To solve this problem must build up the particularity index that can predict the postoperative recurrence or metastasis and further evaluate the treatment result.Currently those original studies are concentrat on early diagnosis and the therapy, but not studies to evaluate its prognosis through finding the tumor marker , the research on recurrence or metastasis of PPC is less .At home and abroad currently ,its research still just the start stage, the beginning see the clues.Glucose transporter protein which mediated glucose uptaked is the main carrier. So far there have been eight kinds of glucose transporter protein (glucouse transporters) identified (Glut1-5 and Glut7-9). Glut-1 is the transit body which is the most widely known .But in normal tissue ,Glut-1 expression confined primarily to red blood cells , the brain, eyes, nerves and placenta.The expression levels of Glut-1 are often very low in other tissue cells. Glut-1 which has been in ovarian cancer, breast cancer and many other tumors showed that the overexpression of Glut-1 have been validated. The overexpression of Glut-1 and tumor development are closely linked. Glucose metabolism provide energy to the process of tumor cells growth.Glut appeared in the almost cell membrane of all cells, and is the main way of glucose into the cells. Cooper R, and others believe that the rapid growth of tumor tissue led to partial reduction of glycogen content and cell hypoxia, resulting in the expression of Glut-1 protein increased, thereby increasing the glucose uptaked in tumor tissue provide energy to the rapid growth of tumor tissueAngiogenesis is the necessary conditions of a solid tumor growth and metastasis ,for the absence of tumor angiogenesis which bring oxygen and nutrients, and remove metabolites, the growth of solid tumor diameter is no more than 2mm. VEGF is now recognized as a specific vascular endothelial cell stimulating factor.It can promote endothelial cell proliferation, promotion of new angiogenesis, while changes in the extracellular matrix caused by increased permeability of blood vessels.In tumor angiogenesis, the endothelial cell proliferation and extracellular matrix degradation are the two basic components.VEGF in the promotion of vascular endothelial cell proliferation and increased vascular permeability is necessary for the tumor angiogenesis .Currently considered, VEGF plays the most basic, critical role in many promoted tumor angiogenesis factors,This paper studies the glucose transporter protein-1 (Glut-1) and vascular endothelial growth factor (VEGF) in pancreatic cancer tissues and normal pancreatic tissue and its significance in pancreatic cancer tissues and normal pancreatic tissue.We investigated the expression of glucose transporter protein -1 (Glut-1) and vascular endothelial growth factor (VEGF) in pancreatic cancer tissues and normal pancreatic tissue. We expect to offer a academic and experimental idea of clinical biological research,early diagnostic,gene cure and estimate prognosis for pancreatic tumor.Objective:To investigate the expressions and significance of glucose transporter-1 (Glut-1) and vascular endothelial growth factor (VEGF) in pancreatic carcinomaMethods:The expressions of Glut-1 and VEGF in pancreatic carcinoma and normal pancreatic tissue were studied with polymerase chain reaction (RT-PCR) and Immunohistochemical stainingResults:RT-PCR results showed Glut-1 mRNA, VEGF mRNA in normal pancreas and pancreatic cancer tissues were expressed, but in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissue (P <0.05); The expression of VEGF showed yellow or brown-yellow granular in pancreatic cancer and mainly located in the cytoplasm of cancer cells and membrane . The expression of VEGF in the cancer cells more visible, while in near cancer and normal tissues the lower. Glut-1 expressed in pancreatic cancer cell cytoplasm and / or the membrane was stained brown-yellow. And normal pancreatic tissues do not express anything. The difference was statistically significant (P <0.05). VEGF expressed stronger around the necrotic tissue in human pancreatic cancer cellsConclusions:1.Glut-1 and the abnormal expression of VEGF may play a synergistic effect in in pancreatic carcinoma2. VEGF expression was related with lymph node metastasis ,but it was not related with tumor grade, clinical stage3. On the one hand ,tumor cells by the high expression of Glut-1 increase glucose uptaked and utilization; on the other hand, a large number of synthesis and secretion of VEGF promote angiogenesis in order to maintain the supply of oxygen and nutrients.Both may participate in occurrence and development of the pancreas cancer4. Glut-1 expression was related with tumor size, clinical stage and lymph node metastasis, but it was not related with pathological grading.