Associated Research About P-selectin Gene Polymorphism And Pyemic Gentic Susceptibility And Secerity

Background and Objective:Sepsis is SIRS that is caused by infection and/or trauma, is an important reason that induce sepsis shock and MODS. Evid by clinical epidemiological data statistics, the important reason that induce patient who suffers from severe trauma, shock or surgery to death is complicating sepsis. Although bacteria strain virulence and amount is the key to induce sepsis, intrinsic factor include sex, age, heredity predisposing genes and so on is very important to pyemic development and consequence. It could be to comprehend pathogenesis about sepsis more scientifically, to bring new chance element for early diagnosis, judgment prognosis and cure to sepsis by investigation the relationship between gene and sepsis. SELP was selected as my experiment object, because it's a important role about WBC initiation adhesion in inflammation and thrombus formation.Methods:Candidate SNP in SELP were confirmed in considering all four factors, including location in gene, association with other diseases, linkage disequilibrium status and minor allele frequency. The experiment object consisted of 255 patients with sepsis and 260 volunteers as control who all from Henan community. GenomeLab SNPstream genotyping platform were employed to genotype. Unconditional logistic regression analysis was performed to analyze the association of polymorphisms with risk and severity of sepsis in five genetic models. P values, odds ratios (ORs), and 95% confidence intervals (CIs) were calculated. We adjusted four confounding factors, including age, sex, smoking-drinking history and chronic diseases history status for analysis of susceptibility to sepsis. In analyzing the severity of sepsis, another confounding factor-APACHEâ…¡scoring was also considered.Results:In the tests of association between SNPs and risk of sepsis, there is significant difference between frequencies of allele C and T of rs3917647 (P=0.013). Frequencies of allele T is higher in control group than case. T allele can diminish the probability of risk of sepsis. In the dominant model, C/T-T/T combination genotype is associated with diminished susceptibility to sepsis (P=0.016) with OR (95%CI) being 0.62(0.42-0.92). Frequencies of allele G of rs3753306 is higher in control group than case (P=0.003). In the dominant model, A/G-G/G combination genotype is associated with diminished susceptibility to sepsis (P=0.0086) with OR (95%CI) being 0.53 (0.33-0.86). Frequencies of allele C of rs2244529 is higher in control group than case (P=0.007). In the dominant model, T/C-C/C combination genotype is associated with diminished susceptibility to sepsis (P=0.01) with OR (95%CI) being 0.61 (0.42-0.89). T-A-C-A-T-C haplotype of SELP is associated with diminished susceptibility to sepsis (P=0.027) with OR (95%CI) being 0.22(0.06-0.84). Single SNP analyzing show all 6 SNPs of SELP isn't associated with severity of sepsis in two genetic models.Conclusion:3 SNPs of SELP are associated with susceptibility to sepsis in dominant model, including rs3917647,rs2244529,rs3753306. T allele of rs3917647 can diminish the probability of risk of sepsis. In the dominant model, C/T-T/T combination genotype is associated with diminished susceptibility to sepsis. G allele of rs2244529 can diminish the probability of risk of sepsis. In the dominant model, A/G-G/G combination genotype is associated with diminished susceptibility to sepsis. C allele of rs3753306 can diminish the probability of risk of sepsis. In the dominant model, T/C-C/C combination genotype is associated with diminished susceptibility to sepsis.T-A-C-A-T-C haplotype of SELP is associated with diminished susceptibility to sepsis.