Evaluation For The Effect Of Pancreatic Kininogenase On Promoting Liquefaction Of Human Semen

Semenogelin I and II and Fibronectin are the major components of human semen coagulum, all originating from seminal vesicle secretions. N-terminal Sg is the inhibition peptide of the whole molecular Sg,which is called SPMI. These proteins are rapidly cleaved after ejaculation by the chymotrypsin-like protease prostate-specific antigen(PSA),which originating from glandula prostata secretions. Zn²⁺ could be important in the regulation of seminal cogulation and liquefaction. Combination of zinc and PSA suppression of its activity, zinc ion release spontaneous activation of PSA. The major Zn²⁺-binding protein are identical with semenogelin I and II. The conformational change induced in semenogelin I by the binding of Zn²⁺ may contribute to the ability of this protein to form a gel ,which is a cohesive network of macromolecular protein complex.Non-liquefied semen is semen coagulation occurred while the gel has not been completely hydrolyzed or not hydrolyzed. When the inhibition peptide(SPMI) not be hydrolysis immediately, spermatozoa motility will be inhibited.In view of the fact that PSA belongs to human kallilrein gene family, which is named human kallikrein 3, and the Pancreatic Kininogenase is a Pancreatic kallikrein extracted from pig pancreas tissue. In this study, to investigate the non-liquefaction semen with the level of Zn²⁺ and PSA and its relationship with sperm motility. To explore Pancreatic Kininogenase whether promote the liquefaction time and improve sperm motility with the low level of PSA in non-liquefaction semen .This paper briefly divided into two parts as follows: 1)Screening 30 cases of patients with non-liquefaction semen and 30 cases of male with normal semen liquefaction. The semens were analyzed by the CASA, and then detected the level of zinc and PSA in seminal plasma, then analyzed and comparised results. Our study explore the semen non-liquefaction with the level of Zn²⁺ and PSA and its relationship with sperm motility. 2)To observe the effect of the pancreatic kallikrein in improving liquefaction time, sperm motility. To seek another effective ways to treatment abnormal semen liquefied and means for abnormal semen liquefaction clinical drug treatment to provide experimental basis. Part I: The levels of Zn²⁺ and prostate specific antigen in the semen with abnormal liquefaction and their relationship with spermatozoa motilityObjective:To investigate the level of seminal zinc and prostate specific antigen in abnormal-liquefaction sperm and its relationship with spermatozoa motility.Methods:Screening 30 cases of patients with non-liquefaction and 30 cases of normal semen liquefaction .The semens were analyzed by the CASA, and then separated from seminal plasma, -20℃preservation.The level of seminal zinc was detected by atomic absorption spectrometry, the level of PSA in seminal plasma was detected by ELISA method, then analyzed and comparised results.Results:Zn²⁺ and PSA of abnormal group were siginificantly lower than those of normal group(P<0.05).The level of seminal zinc of abnormal group is (52.50±0.72)μg/ml,which is siginificantly lower than this of normal group(102.43±0.52)μg/ml (P<0.05). The level of seminal PSA of abnormal group is (0.68±0.14) mg/ml,which is siginificantly lower than this of normal group(1.21±0.21) mg/ml(P<0.05).Sperm motility of abnormal group is siginificantly lower than those of normal group(P<0.05).Conclusion: The levels of seminal zinc and PSA in the semen with abnormal liquefaction is decrease and inhibit the sperm motility. Part II The effect of Pancreatic Kininogenase on Promoting liquefaction of human semenObjective: To investigate the Pancreatic Kininogenase whether promoting liquefaction of human semen and increase sperm motilityMethods: Patients with abnormal from3 to 7 days abstinence, masturbation sperm will be placed in 5 ml of clean dry measuring cup. Each semen specimen were divided into A, B, C three groups, each contain 1ml semen, A group of enzymes are not agents for the blank group, B group kininogenase pancreatic enzyme 40 U, C group urokinase enzyme 5000 U, are placed inside the 37℃constant temperature aqueous bath to observe the three groups of CASA, and liquefaction time.Results: Application of enzyme dose group after the intervention of abnormal semen specimens, semen liquefaction time and significantly reduced sperm motility, the velocity of the blank group were significantly improved. Pancreatic kallikrein and urokinase have good results, pancreatic kallikrein results slightly better than the uPA, but there was no significant difference. Sperm motility of A% is siginificantly increased (15.3±6.5)% than those of blank group(P<0.05).Conclusion: Pancreatic kallikrein can be hydrolyzed and promoting liquefied semen to enhance sperm motility, which would be used as the treatment of non-performing new targeted drug.