Experimental Research On Effects Of Rats' Sciatic Nerve About Stop Training, Reduction Of Training After Heavy-load Swimming Training

Objective: At present, Recoveries of sciatic nerve injury studies have been largely concentrated in the treatment of drug or material, Exercise on recovery of sciatic nerve injury is also unusual. This article was first established by heavy-load swimming training in rat sciatic nerve injury in experimental models, The swimming movements can produce injury to sciatic nerve. Swimming and loads generated by large sciatic nerve injury in training and reduced training adopt the cessation of the movement pattern was observed to explore the two forms of exercise on the impact of sciatic nerve injury, To stop training, reduced training in sports practice, the correct and rational use of scientific training to provide theoretical and experimental basis, but also for the impact of different training on the sciatic nerve and provide a theoretical reference.Research Methods: An incremental load method of establishing a large load of swimming, swimming training animal models, 7 weeks after the rats killed as part of heavy load to observe the load swimming training on the effects of sciatic nerve; Then stop the heavy load group was divided into training groups, and reduced training group, establishing a large load stop after exercise training, reduced training animal model. With biochemical and enzyme physiochemical method of detection of serum creating kinas (CK) activity in serum malondialdehyde (MDA) changes; Using transmission electron microscopy and light microscopy to observe morphological changes in the structure of sciatic nerve reduction; using immunohistochemical staining and computer image analysis method to detect sciatic nerve growth factor (NGF) protein expression in.Research Results1. Training 7 weekend, Compared with the control group, high load growth rate of rats body weight decreased significantly, there are significant differences.(P<0.01);Heavy load of serum MDA activity increased, and the significant difference (P<0.01) , high load group sciatic nerve NGF expression on the mean optical density value of Juncheng rally, and the difference was significant (P<0.01)Sciatic nerve structure, and the control group sciatic nerve myelin sheath structure, clear and complete, which was concentric lamellar structures in close order, high electron density, axons can be seen inside the circle or elongated mitochondria and microfilaments, Schwann cell part of the film package the formation of myelin sheath around the remaining cell body can be seen in mitochondria and lysosomes, such as cell-shun, blood vessels are structurally sound. 7-week heavy load training was found significantly increased cross-section of sciatic nerve, there segmental demyelization, vacuoles, and balloon-like degeneration of injury, axonal swelling and internal structure, electron density to reduce swelling of mitochondria, a serious, we can see the newborn nerve fibers by Schwann cell plasma membrane surrounding the formation of myelin, a slight thickening of the vessel wall.2. Training in 13 weekends, T rats showed an upward trend in body weight, D group also has increased, but the reduction during the training period, T group body weight are always higher than D group, and the difference was significant(P<0.05);Stop training 6 weeks, serum CK activity was significantly lower(P<0.01), serum CK stop training to eliminate slower than reduced training group; there was a significant difference between Heavy load group and stop training group, reduced training group(P<0.01,P<0.01). However, MDA was higher than stop training group reduced training group; Each group of rat sciatic nerve NGF expression changes of the immune reaction products, large load group showed NGF immunoreactivity was stronger than the control group, NGF reduced training ? group showed higher activity of the immune response and large-load group, and stopped near the training group the control group level; Sciatic nerve structure, and stopped training for 6 weeks myelin sheath close to normal, mild swelling of mitochondria can be seen within the axon and microfilaments, vascular endothelial cells were normal. Reduce the heavy load of training tips to stop the nerve damage caused by the movement, so that neural structures gradually restored, but the structure can lead to nerve atrophy, and stopped training longer, there may be neurological degeneration. It can lead to nerve conduction blocked axoplasmic transport obstruction, degeneration of motor nerve endings occur. Reduction training for 6 weeks, myelin structure is normal, demyelization phenomenon is not obvious, showing that Schwann cell nuclei rich in euchromatin, organelles rich, clear and visible structural integrity of vascular endothelial cells in normal. Its structure is better than training in the same period in the control group and the medium-load group. Indicates that large load exercise training reduced exercise capacity, more conducive to the recovery and function of neural structures play.Conclusions1. This paper has established the sciatic nerve injury model of large load training successfully.2. Heavy load swimming can cause sciatic nerve injury in rats.3. Heavy load exercise on rat sciatic nerve caused by injury, and stopped training 6 weeks leading to nerve atrophy and functional decline.4. Heavy-load exercise on rat sciatic nerve caused by injury, reduced training, and organizational structure is conducive to six weeks recovery.