| Objective: High-fat diet is the main factor resulting in overweight, obesity, obesity, diabetes, etc. However, the Insulin Resistance (IR) is the significant factor to induce the diabetes and some other harmful diseases. As we know, aerobic exercise could effectively prevent obesity and relieve IR. So just for the unreasonable diet and reduced exercise, more and more diseases resulted from obesity appear and increase rapidly. For the diseases like IR, exercise and reasonable diet is two mainly effective treatments. The exercise could improve the body sensitivity to insulin and enhance hormonal regulation.In this paper, we mainly research the influence of exercise and high-fat diet on rat's cardiac function which could provide new approach to prevent and treat diseases.Methods: We use 120 1-month-old SPF male SD rats, with the weight of 70±15g to do the research. We control the temperature and humidity respectively from 22°C to 24°C and from 40% to 60%, and use the dry-feed to feed the rats. After feeding one week, we begin our experiment. First, we random divide the rats into normal control group and high-fat diet group. Then, the normal diet group is divided into normal control group (NC) and normal swimming Group (NE), and the high-fat diet group is divided into IR control group (IC) and IR swimming Group (IE), all being fed a total of 15 weeks. NE and IE Group are fed 14 weeks with moderate intensity aerobic exercise. Feeding with high-fat diet after 14 weeks, that is, at the 15th weekend till the final swimming exercise finish, we detect whether the model is successful. Then we determine the rats'cardiac function with the Powerlab testers. Finally we use the automatic biochemical instrument to determine total cholesterol, triglycerides, free fatty acid and other biochemical indexes.Results:①After swimming 14 weeks, the weight of NC group is significantly higher than NE group's (P<0.05), and the weight of IC group is significantly higher than IE group's (P<0.01).②Through measuring FBG and FINS, we calculate ISI index of the rats. The ISI index of NC Group is significantly less than NE group's (P<0.05), and the ISI index of IC Group ISI index is significantly less than the IE group's (P<0.01).③TC of IC Group is significantly higher than NC group's (P<0.01), and TC of IE Group is significantly higher than NE group's (P<0.05). Moreover, TG of IC Group is significantly higher than NC group's (P<0.01), and TG of IE group is significantly higher than NE group's (P<0.01). The FFA of IC group is higher than both NC and IE groups'(P<0.01).④The SOD of NE group is significantly higher than both NC (P<0.01) and IE (P<0.05) groups', and IE group's is significantly higher than IC group's (P<0.01). The MDA of IC Group is significantly higher than the NC group's (P<0.01), and NC group's is significantly higher than the NE group's (P<0.05), also NE group's is significantly higher than the IE group's (P<0.01).⑤The HR and LVEDP of IC group are significantly higher than the NC group's, but the LVSP and±dp/dtmax of IC group are significantly lower than the NC group's. The HR and LVEDP of NC group are significantly higher than the NE group's, but the LVSP and±dp/dtmax of NC group are significantly lower than the NE group's. The HR and LVEDP of IC group are significantly higher than the IE group's, but the LVSP and±dp/dtmax of IC group are significantly lower than the IE group's.Conclusions: High-fat diet can induce insulin resistance, and oxidative stress status has close relationship with insulin resistance, while aerobic exercise can reduce the insulin resistance to a certain extent. High-fat diet can cause insulin resistance of heart, and greatly influence cardiac function, while aerobic exercise can well improve the rat's cardiac function.
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