The Of Tongmai Particles On The Rabbit After Ptca Restenosis And Related Inflammatory Factors

Purpose:Modern medical research has shown that PTCA postoperative restenosis closely related with inflammation, traditional medicine thinks PTCA postoperative restenosis pathogenesis total genera and blood stasis, both the sputum turbidity areas. We by expelling turbidity phlegm legislation, to remove turbidity expectorant effect with the classical prescription of radix soup for large cutting, made from grain, TCM unclogged arteries from protein, genes, cellular level, use the modern cell biology, molecular biology and immunology experimental methods observe unclogged arteries granules on high-fat diets rabbit restenosis after angioplasty and inflammatory reaction related factor matrix metalloproteinases - 2 (MMP - 2), matrix metalloproteinases inhibitors TIMP - 1, discusses the influence of Chinese herbal compound resistance to dispel turbidity phlegm restenosis after angioplasty mechanism of action, for TCM prevention PTCA postoperative restenosis provides a new theory basis, while also widened the big entrapement soup range of applications.Material and method:Healthy male New Zealand big white rabbit 46 only, weight 2.3 kg, plus or minus 0.4 all experimental animals adapt to the environment 1 week. Randomly 10 only as normal group, in ordinary feed more high-fat feeding rabbit. Feed eight weeks. Do iliac artery PTCA resection. Were randomized into control, model group, western medicine, Chinese traditional medicine group, combining group groups of 10 only. After dosing around the end of experiment. By histopathological technology observation at luminal stenosis degree and the pathology changes, Adopt RT - PCR method in detecting rabbit injuried arteries MMP - 2 and TIMP - 1 gene expression in, Using immunohistochemistry methods detection iliac artery MMP-2 damage and TIMP-1 of protein expression. Using Western imprinting method to detect cultivation of supernatant fluid MMP - 2, TIMP - 1 of the protein expressionResults:1.Compared to the normal group, model group animals lumen.2.Unclogged arteries particle group compared with model group, and significantly reduce luminal stenosis, its differences with statistical significance.3.unclogged arteries particle group compared with model group reduce MMP - 2 gene and the protein expression. Meanwhile TIMP - 1 gene and elevated protein expression, the difference was statistically significant.4.Unclogged arteries cell proliferation and apoptosis particle group compared with model reduced significantly, and the serum groups was statistically significant differences.Conclusion:1.With iliac artery balloon damage rabbit with high fat feed method to establish the experimental restenosis after angioplasty model can make thickened, and human endometrium PTCA postoperative restenosis phase approximation.2.Unclogged arteries particles that can reduce experimental PTCA lumen restenosis after the degree.3.Unclogged arteries particles can influence MMP - 2 and TIMP - 1 the expression level between the control, this is one of the mechanism PTCA postoperative RS.4.Unclogged arteries granules contain medicine serum can significantly inhibit inflammatory cells cell proliferation, reduce cell apoptosis rate total.