| This research investigates the effect of cell adhesion molecules ICAM-1 P-selectin protein in the ischemia brain after CI to research the mechanism of early acupuncture treating anti-inflammation injury after CI. MethodsSD rats were randomly divided into sham operation group,model(ischemia 0. 5h reperfusion 6 hours), model(ischemia 0.5h reperfusion 24 hours),model(ischemia 0. 5h reperfusion 72 hours), electroacupuncture treatment(ischemia 0. 5h reperfusion 6 hours, treating 1 time),electroacupuncture treatment(ischemia 0. 5h reperfusion 24 hours, treating 2 times), electroacupuncture treatment(ischemia 0. 5h reperfusion 72 hours, treating 4 times) and the normal control group. Used the suture in MCA to establish MCAO ischemia-reperfusing model. The remarks of nerve function deficit referring to Lundy remarking standards. Used immunohistochemistry SABC(spreptavidin-biotin-peroxidase complex) method to investigate the expression of ICAM-1 , P-selectin in rats' ipsilateral cortex and ipsilateral basal ganglia after ischemia-reperfusion and electroacupuncture. Results1. MCAO and reperfusion injury can distinctly do harm to rats' activity; electroacupuncture can prominently improve its activity after ischemia.2. The expression of ICAM-1, P-selectin in rats' ipsilateral cortex and ipsilateral basal ganglia increases after ischemia-reperfusion.3. Then electroacupuncture can make the expression of ICAM-1 , P-selectin in rats' ipsilateral cortex and ipsilateral basal ganglia decrease.Conclusion1. Releasing the cell adhesion molecules to injury the brain issue after the cerebral ischemia-reperfusion.2. Electroacupuncture can make the expression of ICAM-1,P-selectin in rats' ipsilateral cortex and ipsilateral basal ganglia decrease, then conduce to the protective actions to the brain.3. The mechanism of early acupuncture treating can effectively intervene the injury of CI, is through the immunal regulation to the inflammation reaction after cerebral ischemia. |
PDF Full Text Request