| Objective:under the direction of traditional Chinese medicine(TCM),combining modern research,to observe and discuss the mechanism of raphani soluble alkaloid on depressurization and protection for early-stage kidney damage of spontaneous hypertensive rat(SHR).Method : 40 SHR were randomly divided into 5 groups: control group, raphani soluble alkaloid groups with high,middle or low dosage respectively, captopril group. correspondingly 8 SHR for each group. blood pressure was tested every two weeks by caudal artery manometry, 8weeks after administration, animals were fasted for 12 hours,then their abdomens were opened, furthermore they were put to death by haemospasia and their kidney artery were cut and put into fixative solution containing 10% formaldehyde .24 hours after fixation, wax section and HE dying were prepared for observing the change of kidney arteriole under light microscope, at the same time , the ratio of thickness to cavity diameter for each section was measured and mean value was calculated. Result: 8weeks after administration, raphani soluble alkaloid could lower the blood pressure of SHE obviously, among which high and middle dosage groups manifested no significant difference from captopril group, while each group of raphani soluble alkaloid showed improvement on kidney arteriole of SHE to some extent .Conclusion : the main mechanism of early-stage kidney damage along with hypertension is hyperactivity of liver-YANG, muddy sputum hindering kidney. So "lowering adverse-rising energy and dissipating phlegm, FU-organ smoothness and turbidity evacuation" should be applied as major treatment method .research has proved that raphani soluble alkaloid has conspicuous depressurization and give protection role to early-stage kidney damage aroused by hypertension. |
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