Changes Of 5 - HT And TPH Contents In Rat Strips After Different Time And Exercise

Aim: This experiment by looking at a different time and load swimming exercise onSD rat striatum serotonin (5-Hydroxyptamine,5-HT) and its key enzymes ofTryptophan hydroxylase (Tryptophan hydroxylase, TPH) content changes. Explorethe different time and load swimming exercise in rat striatum5-HT and TPH andlikely impact mechanism, in order to study the mechanism of exercise-inducedcentral fatigue to provide some basis of neurobiology.Methods:66adult male SD rats, fed adaptability and were divided into11groupsafter a week randomly. Blank control group (group C), One-time exhaustion exercise-instantly based group (O1), One-time exhaustion exercise-24hours based group(the O2group), Medium weight training for four weeks-instantly drawn aroundgroup (group EM4-1),24hours around medium weight training materials group(group EM4-2), Around heavy load training-based group (group EB4-1),24hoursaround heavy load training-based group (group EB4-2),Seven weeks of mediumweight training-immediate based group (group EM7-1), Seven weeks of mediumweight training-24hours based group (group EM7-2), Seven weeks of heavy loadtraining-based group (group EB7-1), Seven weeks of heavy load training-24hoursbased group (group EB7-2). Groups in different times after motion beheaded basedand4%neutral formaldehyde fixed all the brain tissue paraffin embedding, in caseof using HE staining and immunohistochemical experiments. With the production ofNikon and SPOT (software for Version3.0) diagram and determination of5-HT andintegral Optical Density value of TPH (Integreted Optical Density, IOD), and thenput the data into Microsoft EXCEL. All the original data use SPSS17.0statisticalsoftware for statistical processing.Results:(1) groups of rats after test weight has increased, and seven weeks of heavyload training group (group and EB7EB7-1-2group) than were significantly higher(P <0.05).(2) in HE staining results, the integrity of the rat striatum neurons formand degree of mesenchymal cells density decreased with increasing of exercise timeand exercise load,24h after motion based and immediately based on morphologicalstructure of rat striatum neurons effect has no obvious difference.(3) in theimmunohistochemical detection, the expression of5-HT and TPH were elevatedwith the increase of the exercise time and exercise load,24h after motion based than immediate based reduced brain5-HT and TPH but not statistically significant.Conclusion:(1)When the rats’ central sports fatigue occurs, the motion area ofcentral nervous system in the striatum neurons active suppression,the content ofstriatum zone5-HT and TPH increases, the change may be one of the causes ofcentral fatigue.(2) medium weight training cuhk rat striate body for a long time, thecontent of5-HT and TPH first fall after rise trend, this trend is conform to the theprocess of the body’s physiological function by to adapt to the changing law of stressin long-term movement;(3) the medium load sports will help improve the body’sability to adapt the exercise-induced central fatigue, and slow the speed of bodyweight growth, tip: medium load sports has an important practical significance toimprove the fitness in national fitness movement,(4) the heavy load exercise andone-time exhaustive exercise make a different degree of damage on the centralnervous system, and no obvious recovery on the central nervous system damage,after24hours rest, tip: one-time exhaustive exercise and large load movementagainst the body fatigue recovery, should not be used in exercise training andexercise for long time, if the body appear in the heavy load exerciseexercise-induced central fatigue, coaches or health instructors should appropriateadjustments to the workout based on the actual situation of activists.