Association Of ACE Gene I / D, CNTF Gene A / G Polymorphism With Scientific Selection Of U - Type Skill Athletes In China

Purposes:In order to seek the relativity between ACE(Angiotensin Converting Enzyme)、CNTF(Ciliary Neurotrophic Factor)gene and the athletic ability of half-pipe snowboarding technique skiers,this thesis use the molecular biology methods analyses the distribution of our country the athletic ability of half-pipe snowboarding technique skiers ACE gene I/D polymorphism and CNTF gene A/G polymorphism, which not only provides reference data for scientific Selection of half-pipe snowboarding technique skiers, but also provides a basis for related exploration and experimental studies. Method:This thesis use 20 excellent China Northern athletic ability of half-pipe snowboarding technique skiersas experimental group, 115 Harbin Institute of Physical Education ordinary undergraduates as control group.The participants are all coming from three northeastern provinces. First we take genes out of the members of the experimental group and the control group.Then we use the method of PCR(Polymerase Chain Reation) determine ACE gene I/D polymorphism of 20 excellent athletic half-pipe snowboarding technique skiers and 115 ordinary undergraduates and use the method of PCR-RFLP(Polymerase Chain Reaction-restriction Fragment Length Polymorphism) determine CNTF gene A/G polymorphism of 20 excellent half-pipe snowboarding technique skiers and 115 ordinary undergraduates.The test results will use Hardy-Weinberg H-W to ensure the demographic representation of testing groups and use Chi-Square test to ensure allele and genotype distribution. Results:1、Subjects in the control group genotype does not conform to Hardy-Weinberg equilibrium(P<0.05), While the ACE gene genotype distribution of excellent of half-pipe snowboarding technique skiers has not fulfilled the balance requirements, the study group does not have a group representation(P<0.05). After chi-square test, The Differences of allele frequency(χ2=0.288) and genotype frequencies(χ2=1.145)were not significant(P>0.05). Allele frequency is I=17.0﹪,D=30.0﹪, and genotype frequency is II=45.0 ﹪, ID=50.0 ﹪, DD=5.0 ﹪ for experimental group; Allele frequency is I=66.7﹪、D=33.3﹪and genotype frequency is II=44.4﹪、ID=42.6﹪、DD=13.0﹪ for control group.2 、 Subjects in the control group genotype conform to Hardy-Weinberg equilibrium(P>0.05), While the CNTF gene genotype distribution of excellent of half-pipe snowboarding technique skiers has not fulfilled the balance requirements, the study group does not have a group representation(P<0.05). After chi-square test, the Differences of allele frequency(χ2=0.032) and genotype frequencies(χ2=1.239) are not significant(P>0.05). Allele frequency is A=17.5﹪, G=82.5﹪, and genotype frequency is AA=1.0﹪, AG=15.0﹪, GG=75.0﹪for experimental group; Allele frequency is A=18.7﹪, G=81.3﹪ and genotype frequency is AA=6.1﹪, AG=25.2﹪、GG=68.7﹪ for control group. Conclusion:1、ACE gene I/D polymorphism can not be uesd as s one of the elite athletes selection index,but ID genotype and I allele account for a large proportion.2、CNTF gene A/G polymorphism can not be used as one of the elite athletes selection index, but GG genotype and G allele account for a large proportion.