Association Of Angiotensin Converting Enzyme I/D And ?-actinin-3 R577X Gene Polymorphism With Physical Fitness Of The Southern Han College Students

Research purposes:Through the analysis of Angiotensin Converting Enzyme(ACE)gene I/D polymorphism and ?-actin-3(ACTN3)gene R577 X polymorphism in southern Chinese han nationality college students in the distribution and correlation with body mass index,so from genetics Angle for genetic markers associated with health.Research objects and methods:This study selected the jiangxi normal university,419 southern han Chinese college students(218 boys,218 girls),all are healthy and ordinary people with no experience in professional sport.This study selected the ACE gene I/D,ACTN3 R577 X polymorphic loci,the application of polymerase chain reaction(PCR)amplification purpose DNA fragments and enzyme digestion and sequencing methods,the analysis of the ACE gene I/D,ACTN3 gene R577 X polymorphism distribution features in research object.At the same time,test subjects related body composition,body function and physical quality index,to explore the correlation of its gene polymorphism.Results:1.ACE gene I/D polymorphism and ACTN3 gene R577 X polymorphism distribution in southern Chinese han nationality college students met Hardy weinberg's law,has a group representative,can be used as a cohort study.2.The ACE gene I/D polymorphism of each genotype in southern China distribution characteristics of the han nationality college students are as follows: II genotype in 162 cases(38.7%),ID genotype in 201 cases(48.0%),DD genotype 56cases(13.3%),along with the Mongolia,Russia and Spain distribution of gene frequency comparison,the results have statistical significance.3.ACTN3 gene R577 X polymorphisms genotypes in southern China distribution characteristics of the han nationality college students are as follows: the CC genotype in 141 cases(35.2%),computed tomography(CT)genotype in 180 cases(44.9%),TT genotype in 80 cases(19.9%),with the north-west China han nationality college students,Jamaica,the African-American population distribution of gene frequency comparison,the results have statistical significance.4.The ACE gene I/D polymorphism in different gender does not appear between body mass index of each genotype in the statistical difference(P > 0.05).5.ACTN3 gene R577 X polymorphism in different gender of each genotype in body shape and body composition index,no statistical difference;Each genotype in female college students physical quality index no statistical difference;The TT genotype in men's physical quality index the sit-and-reach result is much better thanthe CC and CT(P < 0.01),no significant difference was found in all other indicators.6.Portfolio analysis,two genes found in the men's(ACE II + ID&ACTN 3 TT)gene sit-and-reach scores significantly better than(ACE II + ID&ACTN 3 CC + CT)and(ACE DD&ACTN 3 CC + CT)genotype,(P < 0.01).Man(ACE II + ID&ACTN 3TT)genotype is a left hand grip strength score significantly higher than(ACE DD&ACTN 3 CC + CT)genotype(P < 0.05).Discussion:1.The ACE gene I/D polymorphism and ACTN3 gene R577 X polymorphism distribution exist between national and regional differences.2.ACTN3 gene R577 X polymorphism loci and the han male college students in southern China was linked sit-and-reach indexes,prompt ACTN3 gene R577 X polymorphism loci may become one of the molecular biomarker physical prediction and intervention,but also need a larger sample size to validate..3.ACE gene I/D polymorphism and combination of ACTN3 gene R577 X polymorphism may be related to the han male college students in southern China sit-and-reach and left hand grip strength quality constitution phenotype.