| Objective:With the advancement of sports molecular biology,especially human DNA genetic marker technology and the development of human genome project technology,more and more scholars are exploring the value of human exercise ability through the study of human genes.This study aims to explore the association between CNTF and PTK2 gene polymorphisms and resistance training effects through experiments,study gene polymorphisms related to resistance training sensitivity,and provide customized,personalized and precise fitness guidance programs in accordance with.Methods:In this study,191 ordinary college students were subjected to resistance training for 12 weeks to test the changes of 12 muscle strength-related phenotypes,including absolute muscle strength,push and extensor strength,explosive strength,muscle thickness,and muscle mass,before and after training.DNA was extracted from the blood of the patients,and the relationship between CNTF and PTK2 gene single nucleotide polymorphisms and resistance training effects was analyzed.Through the chi-square test of gene frequency and allele frequency,it is verified whether it conforms to the Hardy-Weinberg equilibrium law.When P>0.05,it is confirmed that it conforms to the H-W law.Paired-sample T test and non-parametric test were performed on the data of index muscle weight,muscle thickness,maximum pedaling force,vertical jump,1RM squat and bench press before and after the training of different genotype subjects;test indicators for different genotypes One-way analysis of variance and non-parametric test were performed on the rate of change.All data processing uses Spss21.0 software.The significance level is defined as P<0.05,and the very significant level is defined as P<0.01.For multiple sites,haplo.em of haplo.stats under the R-platform is used for haplotype analysis.haplo.score calculates the relationship between haplotypes and the initial values of physiological indicators and training effects.Haplo.in haplo.stats under R platform is used for haplotype analysis,haplo.score calculates the relationship between haplotype and initial value of physiological index and training effect.Result:(1)After 12 weeks of resistance training,the isokinetic peak flexion strength(2426.89±1067.31→3291.68±1196.88)and the relative maximum power of vertical jump(21.04±6.56→24.31±6.86)of subjects with AG genotype at position rs1800169 of the CNTF gene were both Significant improvement occurred(P<0.01);the peak isokinetic flexion force(2802.34±1104.39→3472.80±1218.89)and the relative maximum power of vertical jump(22.20±6.81→24.46±6.66)of subjects with GG genotype all appeared Significant improvement(P<0.01);comparison between genotypes,the rate of change of isokinetic peak force is AG(0.69±1.85)>GG(0.31±0.37),and the rate of change of vertical jump relative to the maximum power is AG(0.39±1.17)>GG(0.13±0.26).(2)After 12 weeks of resistance training,the isokinetic peak force of flexion(2802.30±1104.4→3472.8±1218.90)and the relative maximum power of vertical jump(22.00±7.00→24.10±7.20)of subjects with AA genotype at position rs948562 of CNTF gene,The thickness of rectus femoris(2.00±0.40→2.20±0.40)all showed a significant increase(P<0.05),and the muscle mass of the lower limbs(8066.70±1898.50→8377.90±1907.00)all showed a significant increase(P<0.01);AG genotype The subjects’peak isokinetic flexion force(2426.89±1067.31→3291.68±1196.88),relative maximum power of vertical jump(21.04±6.56→24.31±6.86),and rectus femoris thickness(1.90±0.34→2.01±0.46)were all significant Sexual improvement(P<0.05),lower limb muscle mass(5471.25±1158.56→8189.81±1955.54)all showed significant improvement(P<0.01);comparison between genotypes,isokinetic peak force of flexion,relative maximum power of vertical jump,thigh The change rate of rectus thickness and lower limb muscle mass was AG>AA.(3)After 12 weeks of resistance training,AA genotype(20.63±5.56→24.73±5.13),AC genotype(21.48±6.48→24.07±6.73),CC genotype(22.66±6.87→24.74±7.00)at rs6994744 of PTK2 gene after 12 weeks of resistance training)The subjects’ vertical jump relative maximum power increased significantly(P<0.01);comparison between genotypes,the relative maximum power change rate of vertical jump was between AA genotype and AC genotype,AA genotype and CC genotype There was a significant difference between the groups(P<0.05),and their AA(0.23±0.19)>AC(0.19±0.65),AA(0.23±0.19)>CC(0.18±0.58).(4)After 12 weeks of resistance training,AA genotype(22.66±6.87→24.74±7),AG genotype(21.48±6.84→24.07±6.73),GG genotype(20.67±5.63→24.67±5.28)at the rs4961288 site of PTK2 gene.The subjects’ vertical jump relative maximum power increased significantly(P<0.01);comparison between genotypes,the relative maximum power change rate of vertical jump was between AA genotype and AG genotype,AA genotype and GG genotype There was a significant difference between the groups(P<0.05),and the AA(0.16±0.56)<AG(0.19±0.65),AA(0.16±0.56)<GG(0.18±0.58).(5)After 12 weeks of resistance training,AA genotype(2.01±0.36→2.14±0.35),AC genotype(1.97±0.37→2.14±0.35),CC genotype(1.91±0.32→2.12±0.4)at the rs10111363 site of PTK2 gene.The subjects’vertical jump relative maximum power increased significantly(P<0.01);comparison between genotypes,the relative maximum power change rate of vertical jump was between AA genotype and AC genotype,AA genotype and CC genotype There was a significant difference between the groups(P<0.05),and the AA(0.74±0.13)>AC(0.09±0.09),AA(0.74±0.13)>CC(0.09±0.11).(6)After 12 weeks of resistance training,CC genotype(2.01±0.36→2.14±0.35),CT genotype(1.97±0.37→2.14±0.35),TT genotype(1.91±0.32→2.12±0.4)at the rs10111852 site of PTK2 gene.The subjects’vertical jump relative maximum power increased significantly(P<0.01);comparison between genotypes,the relative maximum power change rate of vertical jump was between AA genotype and AC genotype,AA genotype and CC genotype There were significant differences between groups(P<0.05),and their CC(0.74±0.12)>CT(0.09±0.09),CC(0.74±0.12)>TT(0.09±0.11).In conclusion:(1)CNTF gene rs 1800169 is related to resistance training effect.AG genotype individuals are sensitive to resistance training and can effectively improve lower limb muscle strength(relative maximum power of vertical jump,isokinetic peak flexion force);rs948562 is related to resistance training effect,AG genotype individuals Resistance training is more sensitive,and it can effectively improve the peak isokinetic flexion force,the relative maximum power of the vertical jump,the thickness of the rectus femoris,and the muscle mass of the lower limbs.(2)CNTF gene rs 1800169 is related to resistance training effect.AG genotype individuals are sensitive to resistance training and can effectively improve lower limb muscle strength(relative maximum power of vertical jump,isokinetic peak flexion force);rs948562 is related to resistance training effect,AG genotype individuals Resistance training is more sensitive,and it can effectively improve the peak isokinetic flexion force,the relative maximum power of the vertical jump,the thickness of the rectus femoris,and the muscle mass of the lower limbs. |
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