Movement And Zinc On The Myocardial Tissue Metallothionein And Its Mechanism Of Action,

Metallothionein (MT) is one kind of metal combination protein that universally exists in persons' and animals' organs .It possesses many kinds of living things effects , and is one kind of strong internal source cell protection matter , it plays an very important role in protecting the blood vessel from injury . Metallothionein can be induced by many kinds of factors, zinc has the closest correlation with it and give play to the organism normal physiology functions.Objective : We inquire into the protective effects and the effect- mechanism of MT on heart ,by way of the influence to MT's content of long-term medium intensity exercise. Method: 40 Sprague-Dawley rats were randomly divided into 4 Groups (n=10, respectively / weighth:220-250g): Group A is control Group, Group B is medium intensity (90min) exercise Group, Group C is exhaustive exercise Group, Group D is zinc +exhaustive exercise Group (10 per Group) .The rats of Group A are fed naturally , and the rats of Group B are carried on the medium intensity exercise (by 8 weeks), and the rats of Group C and Group D are fed naturally , we make the rats of full 8 weeks only do exhaustive exercise for once to measure the content of MT , the levels of (MDA ) and content of -SH , content of Ca2+ , the activity of glutathione peroxides( GSH-px) and Na+-K+-ATP's enzyme in rats myocardial in this study. Result: 1.Compared with the central Group , content of MT was obviously increased in rats myocardial of medium intensity exercise group ( P<0.05 ) , content of MDA and total of calcium capacity were obviously decreased ( P<0.05 ), the activity of glutathione peroxides( GSH-px ) and Na+-K+-ATP's enzyme were obviously increased ( P<0.05 ) , content of - SH had no significant difference , but it had experienced a obvious increase.2.In exhaustive exercise Group, content of MT in rats myocardial was notably reduced ( P<0.05 ) than central Group , content of MDA and total of calcium capacity were obviously increased ( P<0.05 ), - SH content and the activity of glutathione peroxides( GSH-px) and Na+-K+-ATP's enzyme were obviously reduced (P<0.05 ). 3.Compared with exhaustive exercise Group, content of MT was obviously increased in rats myocardial of zinc +exhaustive exercise Group (P<0.05 ) , content of MDA and total of calcium capacity were obviously decreased ( P<0.05 ), content of - SH and activity of glutathione peroxides( GSH-px ) and Na+-K+-ATP's enzyme were obviously increased (P<0.05 ).Cnclusion : ( 1 )Long-term medium intensity exercise can induce MT's adaptabilityincreases in rats myocardial, raise myocardial ability of resisting oxide . It results in MTreducing MDA's content in the myocardium and preventing total calcium capacityincrease as well as improvement GSH-px and Na+-K+-ATP's enzyme activity.2 ) Exhaustive exercise can make the content of MT descend .It may has relation withthe following factors: numerous inner resource free radicals which emerge in process ofexhaustive exercise , its content surpasses MT's generating and the content of otherresisting oxidize enzyme greatly, creating cardiomyocytes injury, MT's self-consumingin course of scavenging the free radicals , causing MT's content reduction.( 3 ) MT and metal zinc are mutually related closely , and they two probably participatein protecting organism organization injury after exhaustive exercise .