| To study the immune function of extracted hydrophilic polysaccharides in Radix Isatidis on the immune organ, system immune and mucous membrane immune of the SD line renewal rats without colostrums and provide theories basis for exploitation of the Radix Isatidia Polysaccharide (RIP) that is green, no adverse effect and no drug residue. Renewal rats were grouped and administrated with different doses of IRP, and used the anatomy technology, flow cytometry. and immunohistochemistry technology to detecte the immune organ indexes, the numbers of T lymphocyte subpopulations in peripheral blood, the development of small intestine, the quantities of goblet cells, mast cells. SIgA and IgG positive cells in the small intestine. The results showed that(1) RIP was no trophicity. It didn't add the body weight of the renewal rats without colostrums on the first 28 days and the small intestinal weight at days 7-21. However, it could significantly raise the small intestinal weight at day 28.(2) RIP could increase the thymus gland index and spleen index of the renewal rats without colostrums. The doses of RIP which had the best effectiveness in the thymus gland and spleen index were decreased by the days. The thymus gland index in group A and the spleen index in group C at day 21 were significantly bigger than others at day 7 (P<0.05)(3) RIP could raise the numbers of CD3+, CD4+, CD8+T cells in the renewal rats without colostrums. The dose of RIP having the best effects on CD3+, CD4+T cells was 20 mg/kg, and on CD8+T cells was increased by the days. The numbers of CD3+T cells and CD4+T cells in group C, D and E were significantly larger than group A in the first 7-21 days (P<0.05). The numbers of CD8+T cells in group C, D and E were significantly larger than group A and B at days 7,21 and 28 (P<0.05)(4) RIP could promote the morphological development to the small intestine of the renewal rats without colostrums.It could enlarge the villi height of the small intestine, lower the crypt depth of the small intestine and increase the value of V/C. The doses of RIP having the best effect on villi height, crypt depth, value of V/C were increased by the days.(5) RIP could increase the quantities of goblet cells in the small intestine of the renewal rats without colostrums. From the duodenum to ileum, the numbers of goblet cells were gradually increased. The doses of RIP having the best effect on goblet cells in the small intestine were increased by the days. The quantities of goblet cells in duodenum in group C were remarkably higher than group A and B at day 7. These cells in jejunum the group D were remarkably higher than others at day 7 (P<0.05) and group C, D and E were remarkably higher than B at day 28 (P<0.05). The numbers of ileum in group C and group D were respectively remarkably higher than others at days 14 and 28 (P<0.05)(6) RIP could increase the quantity of mast cells in the small intestine of the renewal rats without colostrums. From the duodenum to the ileum, the numbers of mast cells were gradually decreased.The best dose of RIP in mast cells of the small intestine was increased by the days. The amounts of mast cells of duodenum respectively in group E. E and D, D were remarkably higher than others at days 7.21,28. (P<0.05) The numbers of jejunum in group E were remarkably higher than group B at days7 and 28 (P<0.05). The numbers of ileum in group C were remarkably higher than others at day 14 (P<0.05)(7)There were sporadic IgG positive cells in enteraden cavity lamina propria in the small intestine in 0 day and no SIgA positive cells were found. IgG and SIgA positive cells were first present in enteraden cavity lamina propria, and located in the villus of lamina propria in the 7-14 days. On the 21-28 days, a great number of IgG and SIgA positive cells were distributed in enteraden cavity lamina propria and villus of lamina propria in the small intestine. RIP could advance the secretion of IgG and SIgA positive cells in the small intestine mucous membrane in the renewal rats without colostrums, and the best dose of RIP on the numbers of IgG and SIgA positive cells was grew down by the days.In a word, the RIP was no trophicity, and it didn't add the body weight of the renewal rats without colostrums. However, it could increase the small intestinal weight, promote the development immune organ, increase the numbers of T lymphocyte subpopulations in peripheral blood, advance the morphological development of small intestine, raise the quantities of goblet cells and mast cells, and boost the SIgA and IgG positive cells in the small intestine. |
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