The Research On The Propagation Techniques Of Euonymus Japonicus Cv. Microphyllus Butterscotch

Euonymus japonicas cv.Microphyllus Butterscotch belongs to Euonymus,is a cultivar of Euonymus japonicas Thunb. Its leaves show a gold and stable color which owns a high ornamental value. Furthermore, due to strong resistance and resistance characteristics of pruning, Euonymus japonicas cv.Microphyllus Butterscotch is an excellent material to construct colored fences, color lumps and mode patterns. It is widely used in America, Australia, Japan and etc. However, the application of this cultivar is rarely reported.To successfully apply Euonymus japonicas cv.Microphyllus Butterscotch to landscape in China, we conducted the study of propagation about Euonymus japonicas cv.Microphyllus from Australia, including tissue culture technology, cutting propagation technology and seed propagation technology. It can be concluded as follows:1. Tissue culture of Euonymus japonicas cv.Microphyllus Butterscotch(1)Selecting the period for collecting data of explantsStem segments with axillary buds are selected to study the influence of three different collecting explants periods on bud induction. The three different collecting explants periods are April, June and August respectively. The result shows that the best time of collecting explants of Euonymus japonicas cv.Microphyllus Butterscotch is June with a contamination rate of 10.2%.(2)The best culture medium of primary culture selection is:MS+NAA 0.1 mg /L+6-BA 0.5mg/L+IBA 0.05mg/L. The induction rate could reach 76.67%.(3) The best culture medium of subculture selection is MS+NAA O.1mg/L+6-BA 0.5mg/L. The bud proliferation factor is 4.50.(4) The best culture medium of rooting culture selection is:1/2MS+NAA 0. 5mg/L+IBA 1.Omg/L. The rooting rate could reach 59.85%2. Cutting propagation of Euonymus japonicas cv.Microphyllus Butterscotch(1)The influence of cutting medium on rootingFour types of medium are chosen to conduct the cutting experimental, including peat:perlite=1:3, peat:Vermiculite=1:1, sand:soli=1:4 and perlite respectively. The result indicates that the mixed medium of peat:perlite=1:3 is most appropriate to the cutting of Euonymus japonicas cv.Microphyllus Butterscotch. The rooting rate of cuttings can obtain 52.08%.(2)The influence of cutting time on rootingCutting experiments are performed in June, July, August and September respectively. The experimental result shows that the best rooting time is July and August. The rooting rate of the two months can obtain 22.91% and 20.83% respectively.(3)The influence of Exogenous hormones on rootingThree types of endocrines are chosen to dealt with on cuttings, namely IBA, NAA and Hormones. Each endocrine are set with three different concentration levels. In IBA and NAA, three levels of 1000ppm,3000ppm,8000ppm are selected. In Hormones, three levels of Horml, Horm2, Horm3 are chosen. With the comparison of different concentration levels, Horm3, IBA3000ppm and NAA1000ppm are the highest rooting rate in each endocrine. In all endocrines, the highest rooting rate is in Homr3.(4)The type of rootThrough observations of root areas in surviving rooting cuttings, roots is grown out of the branches'skin with no Root callus. The root of Euonymus japonicas cv.Microphyllus Butterscotch is Root cortex.3. Seed propagation of Euonymus japonicas cv.Microphyllus Butterscotch(1) Cold stratificationCold stratification can effectively break seed dormancy to improve the germination rate. As the time of cold stratification increases, the seed germination rate and germination potential show a gradual growing trend. When the time of cold stratification reaches 60d, the seed germination rate can obtain 64.67%.(2) GA3 soaking treatment after cold stratificationCold stratification and GA3 soaking treatment have a great influence on seed germination rate. When 500ppm GA3 soaks seeds for 4d after 60d of cold stratification, the seed germination rate can achieve 81.33%.(3) GA3 soaking treatment before cold stratificationGA3 has a great influence on seed germination rate. When 500ppm GA3 soaks seeds for 4d before 30d of cold stratification, the seed germination rate can obtain 71.33%.4. The study of Adaptability on Euonymus japonicas cv.Microphyllus Butterscotch With a year of adaptability on Euonymus japonicas cv.Microphyllus Butterscotch, a preliminary result shows that Euonymus japonicas cv.Microphyllus Butterscotch has both strong cold resistance and heat resistance to survive in both summer and winter of Changsha. It can be concluded that Euonymus japonicas cv.Microphyllus Butterscotch can grow normally in Changsha. There are few variations in the leaves of seedling transplanting cuttings. The color of their leaves mostly remains golden. The genetic color of the leaves of tissue culture stays stable. The coefficient of variation of seedlings is high and 45.3% of plants have green leaves.With comparisons of different Euonymus japonicas cv.Microphyllus Butterscotch propagation approaches, it can be concluded that:the Breeding cycle of tissue culture is short; The optimal regeneration system of Euonymus japonicas cv.Microphyllus Butterscotch is Initially established by selecting optimal Medium hormone combination. Tissue culture can obtain larger number of seedlings of Euonymus japonicas cv.Microphyllus Butterscotch in the short time, which provides technic support for factories to grow seedlings. The root cutting propagation rate is high and the color of leaves in plats is stable, which can be used for factories to grow seedlings. The seed germination rate is high after treatment, whereas the coefficient of variation is high, which can't keep the original fine features of plants. If it needs the large-scale production of breeding, clone is recommended to be adopted with high propagation coefficient and Genetic stability.