| Gerbera jamesonii is one of the most important cut and pot flowers worldwide. Numerouscultivars have been released as cut flowers, pot flowers, or garden plants. In China, the acreagedevoted to this crop continues to increase. But in China, the cultivated varieties of G. jamesonii arestill relyng on import and cultivated varieties less. So the main goal of this work was to create newgermplasm by combining the method of chemical mutagenic treatment technology and tissueculture technology for laying the foundation of the germplasm innovation. The main contents areas follows:1. In vitro culture system of G. jiamesonii has been founded.Make young receptacle of G. jiamesonii as explants, the best sterilization treatment is usingof0.1%HgCl2for12minutes, the best callus induction medium is: MS+6-BA8.0+NAA0.5for‘Rosalin’‘Champagne’ and ‘Linda’, MS+6-BA8.0+NAA0.2for ‘High Mountain’, MS+6-BA8.0+NAA1.0for ‘Franlunce’, MS+6-BA5.0+NAA0.1for ‘Red Star’, the bestmultiplication induction medium is: MS+6-BA0.5+NAA0.1for ‘Rosalin’, MS+6-BA1.5+NAA0.1for ‘Champagne’, MS+6-BA1.0+NAA0.1for ‘Linda’ and ‘Franlunce’, MS+6-BA1.5+NAA0.1for ‘High Mountain’,‘Red Star’ couldn’t redifferentiation in all the multiplicationinduction medium, except for browning reaction and even death. The whole varieties of Gerbera’best rooting medium is1/2MS+NAA0.1.2. Research on the parts of G. jamesonii by chemical mutagenesis treatmentWith DES is0.1,0.2,0.4,0.6and1.0%concentration and0.5h treatment, the result showedthat: a week precultured receptacle couldn’t induct to form callus, the callus all most becomebrowning, or even death, not for proliferation, while dwarf stem has a little of browning and deathphenomenon, many of them could continue to multiply in a short time, suitable for mass formutagenesis, screening specific mutants.3. Research on the half lethal dose of the different chemical mutagenesisThe semi–lethal dose of DES is0.5％concentration and50min treatment, EMS is0.8%concentration and4h treatment, SA is0.2%concentration and1h treatment. And the death rateof sodium azide treatment increased with concentration increasing is the maximum, the changerange of EMS is the minimum, and the DES is the middle of them.4. Effects on the growth of in vitro propagation of G. jamesonii by different mutagens The effects of the growth rate of DES is after the first fast slow with the concentrationincreased, both EMS and SA become slowly with the concentration increased. In the lowconcentration of mutagenic treatments, different mutagens all have stimulated the dwarf stem toproliferation more than normal circumstances. With the mutagenic treatments dose increased, thenumber of the multiplication decreased of all the mutagens until not proliferation. In0.3%concentration and50min DES treatment, the proliferation seedlings become short and strong, andgrow well, while in a high concentration of conditions, the proliferation seedlings’ leaves becomethinning and have some spotted; in low concentration of EMS treatment, proliferation seedlingsgrowth well, sporadic appear white spots, while in high concentration, proliferation seedlingsquality is poorer, and more spots in leaves; The SA mutagenesis treatment, except for0.05%concentration of the proliferation of G. jamesonii seedling quality the impact is not big, the rest ofthe concentration have more influenced of proliferation seedlings, and at0.1%concentration, theproliferation of seedlings leaves will present a growth bad phenomenon, but also some leaf willgradually wither in training, increasing concentration will have the vitrification seedlings appear,and proliferation seedlings become poor quality.In this article, in vitro propagation of G. jamesonii has been established. And combined themethod of chemical mutagenic treatment technology and tissue culture technology on the breeding,the benefit to the preservation of the germ plasm resources in G. jamesonii. and develop andutilize it. In this subject that lays a ground work for the breeding of G. jamesonii by chemicalmutagen. |
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