The Preliminary Study On The Bt Rice Effect On Growth And Reproduction Of Spiders(Pardose Pseudoannulatd)

Abstract:The ecological safety problems on transgenic anti-insect crops are highly valued by the governments and people all along. This paper focus on Bt rice and its dominant pests predator’s Pardosa pseudoannulata to discusse the regulation of Bt protein expressed by Bt rice in the food chain of Bt rice-rice planthopper-Pardosa pseudoannulata and to ascertain the effects of the Bt protein on Pardosa pseudoannulata’s growth, development and reproduction at its third level triphic level. The main reseatch results are as follows:1. The enzyme-linked immunosorbent assay (ELISA) was used to investigate the Cry1Ab protein in the Pardosa pseudoannulata body. Then we found that Cry1Ab protein transferred along the food chain of Bt rice-rice planthopper-Pardosa pseudoannulata, where the protein enrichment existed. The Cryl Ab protein in the rice planthopper increased with feeding time extension, reached the peak at the7th day with a enrichment coefficients of24.38, and then the content gradually reduced.2. We determined Pardosa pseudoannulata’s growth and development indicators, including the development duration, weight at several instars, consumption, survival rates, the organs development and so on. Data of the development duration showed that the experimental group (73.21d) was longer than the control’s (67.23d). This comes to a conclusion that the Bt protein may lead to a hysteresis on Pardosa pseudoannulata development duration so that the development duration of the experiment group was significantly longer than the control’s and its weight was less than that of the control group significantly.3. The spiderlings matured and then mated to explore the effects of the protein on the spider’s reproduction. Results indicated that the protein had an insignificant impact on the spawning bag and yolkgranules number in their spawning prophase.4. Spider blood samples was extracted for the related blood biochemical indicators. Meanwhile, we applied the flow cytometry to analyze the changes of spider’s cell apoptosis and calcium currents. All the data confirmed that Bt protein had nonsignificant effects on larval and adult spiders’s blood.In conclusion, our study demonstrated that:the Bt protein expressed by Bt Rice can transferred in the food chain and be enriched in secondary consumer. Although there were no adverse effects to spiders after fed with Bt protein in the trial period, their development duration was delayed distinctly. This may result from the spider’s related molting hormone adjustment by Bt protein. Therefore, we need further research about the effects of Bt protein on spider’s related hormone regulation.