| The test use160Duroc crossbred growing piglets consistent with similar weight and health, which were randomly divided into I, II, III, IV group, n=5replicates, each repeated group has8piglets, respectively, was fed the basal grain, basal diet+Colistin sulfate50mg/kg+Chlortetracycline75mg/kg, the basal diet+0.5%compound Chinese herbal medicine, the basal diet+1.0%compound Chinese herbal medicine; the pre-test was10d, the positive test was30d;in the test period take the relevant records, at the end of the trial, gets the samples and detect indicators for statistical analysis;in order to study the compound Chinese herbal how impact the piglets growth performance, intestinal enzyme activities, intestinal structure, and immune effect;and compared with antibiotic group in order to explore the feasibility of using this compound Chinese herbal to alternative the antibiotics in growing piglets; This test consists of three parts:Test one:compound Chinese herbal medicine on the performance of growing pigletsDuring the test, record growing piglet feed intake; the beginning and the ending of the experiment growing piglets weighing, calculating the average daily weight gain, average daily feed intake and feed conversion ratio (feed conversion); And recorded the number of diarrhea piglets and diarrhea days, and then calculated the rate of diarrhea;the experimental data were statistically analyzed,and the results showed that:the compound herbal medicine additive played a role in promoting the growth of growing piglets, the growing piglets average weight gain and feed intake has increased, but the difference did not reach significance (P>0.05). Test Group III and Group IV day average weight gain compared with the control group were increased by2%;The test IV group than the control group I increased by2%for average daily feed intake, rather than the Test Group II improved1%. For all:on the feed conversion, test II, III, IV, group have shown a downward trend relative to the control group and test group IV reached the lowest. Experiment II, III, IV group diarrhea rates were lower than the control group I, respectively38%,55%and58%.Comparing with the group Ⅰ.the group Ⅲ was significantly lower (P<0.05);Other groups had no significant difference;group III, IV and the group II was no significant difference (P>0.05), but got a decreased trend.Test two:compound Chinese herbal medicine on the impact of growing piglet intestinal structure and intestinal activity At the end of the trial, each repeating group randomly selected a healthy piglet, slaughter, then taken duodenum,jejunum and ileum’s intestinal contents, and scissor duodenum, jejunum, ileum2cm spare, sample processing then detection analysis; the results showed that: experiment II, III and Group IV’s bowel villus height were highly significant differences from control group (P<0.01), crypt depth in each experimental group in tandem with the villus height increased and became larger, the difference was extremely significant (P<0.01), but the ratio of villus length and crypt depth, the test group than the control group were increased, which, the test group IV duodenum, ileum and Test Group III of the jejunum was significantly higher (P<0.05); Compared with the test group II (antibiotic group), Test Group IV of the duodenum, ileum and test Group III ileum villus height very significantly improved(P<0.01), although the intestinal villus length and crypt depth ratio was not significantly different in the experimental group III and IV and the antibiotic group (P>0.05), but there is a tendency to increase.The effect of the compound Chinese herbal additives and antibiotics in different parts of the intestinal amylase activity was not significant, the experimental group Ⅲ in the duodenum was significantly higher (P<0.05)than the control group, other intestinal segments of each experimental group compared with the control group and experimental group is not significant (P>0.05). Add the compound Chinese herbal additives can improve pig intestinal activity of trypsin, and the results are better than antibiotics experimental group. Adding different proportions of compound Chinese herbal medicines and antibiotics for weaned piglets, the gastrointestinal lipase was significantly different (P<0.05), and adding0.5%compound Chinese herbal trial group had the best effect.Test three:compound Chinese herbal mixture immune function in growing pigletsAt the end of the trial, each repeating group selected a healthy piglet,then took blood from the ear vein, and spared serum, centrifugal separation and determined swine fever antibody titers, serum immunoglobulin IgG, interleukin-6(IL-6) and tumor necrosis factor (TNF-alpha).The results showed that: the difference of the classical swine fever antibody levels of group III and group I compared with the control group were significantly (P<0.05); the difference between the other groups was not significant (P>0.05). Compared with the group I, the test group II piglet’s serum IgG level was significantly improved (O<0.05);but the IL-6and the TNF-alpha were not significantly (P>0.05); the group III and group Ⅳ piglet’s serum IgG and TNF-a had a significant increase (P<0.05), IL-6was very significantly lower (P<0.01). Compared to group II,the group III and group IV trial of IL-6was significantly lower (P<0.05); serum IgG and TNF-α were not significant difference, while had a rising trend.The results:the compound Chinese herbal additives added in the basal diet of growing piglets, has improved the performance of pigs, and promoted the rapid and healthy growth of the growing piglets; this compound Chinese herbal medicine can improve the activity of the intestinal tract of growing piglets, to promote digestion and absorption of nutrients; can effectively improve the growing piglet small intestine villus height, crypt depth, increase the ratio of villus height and crypt depth; can effectively protect the integrity of the intestinal structure,the intestinal thickness of each experimental group was no significant difference;It can improve the growing swine fever antibody levels to improve their vaccine immune responses. The Chinese herbal medicine group and the antibiotic group difference was not significant, results showed that the compound Chinese herbal additives is one of the ideal substitute for antibiotics. |
PDF Full Text Request