Studies On Chemical Constituents Of Clematis Manshurica Rupr

Clematis manshurica Rupr. belonging to the family Ranunculaceae is commonly used astraditional Chinese medicine Wei Ling Xian to get rid of rheumatoid and pass the meridian. Atpresent，about60compounds has been isolated from the root of C. manshurica Rupr., thesecompounds contains saponins, alkaloids, lignans, phenolic glycoside, lactones and organi acid etc.There were little studies about phenolics contents and essential oil of C. manshurica Rupr.. In thispaper, the chemical components of C. manshurica Rupr. were systematically researched，includingpurification and identification of some compounds, comparison mass fraction of phenolics contents ineach extract fraction of C. manshurica Rupr. and determination of chemical components of essentialoil from C. manshurica Rupr.. Experimental results are of benefit to define chemical constituents of C.manshurica Rupr. and to better use of this medicinal plant.Frist, five compounds, β-sitosterol(1), stigmasterol (2), epifriedelanol (3), friedelin (4), anemonin(5) were isolated by column chromatography from the dry roots of C. manshurica and their structureswere identified on the basis of physicochemical properties and spectral data. Compound3and5wereobtained from this plant for the first time.Second, mass fraction of phenolics of C. manshurica Rupr. were determined by spectrophotometrywith rutin as the criterion caliber. Rutin standard curve regression equation is y=9.516x-0.0233, relatedcoefficient R2=0.9997. Mass fraction of phenolics in crude methanol extracts, ethyl acetate fraction,n-BuOH fraction and water fraction were compared.The results showed that ethyl acetate fraction hadthe highest mass fraction of phenolics, the mean was83.20mg/g, n-BuOH fraction, crude methanolextracts and water fraction were in turn.Third, the chemical components of essential oil of C. manshurica by steam distillation, theessential oil was analysed by GC-MS, the amount of the chemical composition of the essential oil weredeterminated by normalization method.207peaks were detected,111compounds were identified,72.87%of the total peak areas. The relative content of84compounds were at above0.1%,71.66%ofthe total peak areas. The main constituents were hexadecanoic acid ethyl ester,9,12-octadecadienoicacid ethyl ester, tetradecane, hexadecane,(E)-6,10-dimethyl-5,9-undecadien-2-one, α,α-4-trimethyl-3-cyclohexene-1-methanol, dodecane,(Z,Z,Z)-9,12,15-Octadecatrienoic acid ethyl ester, octadecaneand phthalic acid butyl hexyl ester.