Expression And Significance Of Interleukin-18and Interleukin-12in Hypercholanaemia Of Pregnancy And Intrahepatic Cholestasis Of Pregnancy

Objective To investigate the clinical significance of interleukin-18,12and tumor necrosis factor-α in idiopathic hypercholanaemia of pregnancy.Methods70cases of pregnant women with GlycocholicAcid≥10.75μmol/L, including40cases of dysfunction of liver (ICP group),30cases of normal liver function (19cases were asymptomaticgroup,11cases were symptomatic), and30cases of normal pregnant women(control group) were recruited. Serum IL-18, IL-12and TNF-α were examined by ELISA. Serum alanine aminotransferase (ALT) and aspartateaminotransferase (AST) were examined by automatic biochemical analysisinstrument.Results (1) In ICP group,serum concentrations of IL-18, IL-12andTNF-α were （129.6±22.9）ng/L,（76.3±21.4）ng/L and （84.5±12.3）ng/L. In normal liver function group, serum concentrations of IL-18, IL-12and TNF-α were（67.8±15.2）ng/L,（31.6±10.2）ng/L and（51.4±10.7）ng/L. While in control group, serum concentrations of IL-18, IL-12andTNF-α were（43.4±13.2）ng/L,（10.2±3.4）ng/L and （32.5±9.0）ng/L.Serum IL-18, IL-12and TNF-α levels in hypercholanaemia of pregnancygroup were significantly higher than those in control group(P<0.05).SerumIL-18, IL-12and TNF-α levels in ICP group were significantly higher thanthose in normal liver function group(P <0.05).In normal liver functiongroup, serum IL-18, IL-12and TNF-α levels between pruritus group and nopruritus group showed no significant difference(P＞0.05).(2) Theincidences of premature birth（60%） and meconium-stained amniotic fluid（30%） in dysfunction of liver group were significantly higher than thosein normal liver function group （23%，7%） and normal controlgroup(P<0.05).The incidences of premature birth(23%) andmeconium-stained amniotic fluid in normal liver function group weresignificantly higher than those in normal control group(P<0.05). While innormal liver function group, The incidences of premature birth between pruritus group (11%) and no pruritus group (27%) showed significantdifference (P <0.05).Conclusion The expressions of IL-18, IL-12and TNF-α betweenidiopathic hypercholanaemia of pregnancy and intrahepatic cholestasis ofpregnancy is different. The maternal and neonatal outcomes inhypercholanaemia of pregnancy are good. Objective To investigate the effect of interleukin-18and interleukin-12in hepatic injury induced in intrahepatic cholestasis of pregnancy（ICP）.Methods Six-two cases of ICP patients(ICP group),30cases ofhealthy pregnant women (control group) and30cases of hepatitis B（HBV）women (hepatitis group) were recruited. Serum IL-18,IL-12and TNF-αwere examined by ELISA. Serum alanine aminotransferase (ALT) andaspartate aminotransferase(AST) were examined by automatic biochemicalanalysis instrument.Results (1) The levels of IL-18, IL-12, TNF-α, AST and ALT in hepatitis group were significantly higher than those in ICP group andnormal control group,the difference was statistically significant (P <0.05);The levels of IL-18, IL-12, TNF-α, AST and ALT in ICP group weresignificantly higher than those in normal control group, the difference wasstatistically significant (P <0.05).(2) The levels of IL-18, IL-12, TNF-α,AST and ALT in severe ICP group were significantly higher than those inmild ICP group and normal control group, the difference was statisticallysignificant (P <0.05); The levels of AST and ALT in mild ICP group wassignificantly higher than those in normal control group, the difference wasstatistically significant (P <0.05); compared with the levels of IL-18andIL-12in mild ICP group and normal control group,there was no statisticallysignificant difference (P>0.05).(3) The morbidity of premature infant andMeconium-stained amniotic fluid in the severe ICP group weresignificantly higher than those in mild ICP group and normal control group,the difference was statistically significant (P <0.05).Conclusion IL-18may be involved in process of hepatic injury ofICP.