Day Lilies Flowers Chemical Constituents And Quality Control Research

Daylily (Hemerocallis fulva) of the Liliaceae is a plantlily buds, also known as daylily as a vegetable and drugs in Asia with a thousand years of history."Compendium of Materia Medica"contains daylily can cure urinea stringent body Fanre, in addition to the wine jaundice; digestion, hot and humid Lee Xiongge, five organs,It is good and joy,worry-free,light-weight names.Modern pharmacological proves daylily flower antioxidant,lipid-loweringeffects, antitumor and neural pharmacological activity.Daylily spends less chemical composition,and antidepressant activity monomer have not been reported.The Hemerocallis is not posted pharmacopoeia,only the industry standard, that makes lots of restriction in clinical application.Therefore,this subject studies daylily alcohol extract of constituents with modern chemical separation techniques and means.According to chemical result,the quality control method was established.(1) Chemcal Constitution StudyTechniques as TLC,normal phase silica gel column chromatog raphy,polyamide column chromatography,sephadex column chromatography etc.hace been used to determine chemical constituents of Hemerocallis fulva;The same times, structures have been identified through making use of physical and chemical properties and spectroscopic methods. A total of13compounds are isolated,9of them are identified.There arerutin, quercetin,quercetin-3-O-β-D-xylopyranosyl, hyperoside, isoquercitrin, β-sitosterol, succinic acid,3-furoicacid, glucose. Four compounds(hyperoside, isoquercitrin, succinic acid,3-furoicacid) were isolated from the plant for the first time.Separation of the nine compounds, through literature review, hyperin has antidepressant effects.Therefore, as further study of daylily quality control method of the index component.(2) Daylily control Study on the basis of the chemical study, the daylily quality control were studied:(1) To hyperin for the index components, establishment of daylily TLC method.(2) In TLC id-entification based on, using HPLC method established hypericin content determination in dalily, and determination of8different clubs in the origin of hyperin content.The results show that Guangdong Hyperoside content is the highest, Shaanxi hypericin content was the lowest, and there are the great difference of the contentsduring different origins.(3) to the content determination method for the foundation, using the method of HPLC established day lily fingerprint, method of inspection results.Using similarity software identifies a batch of10samples of11common fingerprint peaks, and3of them were identified (both Flavornoids).In summary, this paper conducted a day lily chemical composition and quality standard research, discovery has antidepressant effects of flavonoids, Hemerocallis flower for antidepressant effects provided corporeal basis.And the establishment of day lily herbal quality standard, for further development and utilization of ramie flower herbs provide a basis.