Miao Medicine Skin Hemorrhoids Ointment To Promote Healing Of Skin Ulcers In Diabetic Rats Qing Mechanism Research

Objective:To observe the effects and mechanism of Fuzhiqing ointment on healing diabetic skin ulcer in rats by detecting the speed the healing of skin ulcers in diabetic rats, granulation tissue fibroblast cell numbers, new capillaries and EGF, bFGF and VEGF levels change, and then make the Fuzhiqing ointment application.Methods:The rat models of diabetic skin ulcers were constructed by which the magnetic sheet was implanted in the right foreleg and STZ was injected into enterocoelia, then,exogenous magnetic sheet was implanted in the right foreleg to compress the blood circulation.45rats were divided randomly into3groups:Fuzhiqing group treated with Fuzhiqing ointment externally, Vaseline group with Vaseline ointment externally,treatment two times a day;and Blank group with nothing. After3days,6days,9days’ measurements in each group randomly kill5rats, measure the wound areas,take the wound tissue in the optical microscope counting groups of granulation tissue fibroblast cell number, number of new capillaries,and with immunohistochemistry detection method and Image-Pro-Plus image analysis systems group in granulation tissue of vascular endothelial growth factor (VEGF), fibroblast growth factor (bFGF), epidermal growth factor (EGF). Results:(1) The wound area of Fuzhiqing group was1.41+0.08,1.01±0.08,0.63±0.07(in cm2),after3,6or9days’ treatment,compared with Vaseline group and Blank group have a significant difference (P<0.05).(2) The histopathological examination show that Fuzhiqing group in3,6or9days’treatment, granulation tissue fibroblast cells were75.6±2.07,127.40±3.44,50.40±2.7;Number of new capillaries24.6±1.14,42.20±1.30,16.80±0.83, respectively, compared with the Vaseline group and Blank group have a significant difference (P<0.05).(3) Immunohistochemical detection show that the Fuzhiqing group in3,6or9days’treatment, fibroblast growth factor (bFGF) AOD values were0.204±0.035,0.148±0.019,0.096±0.015, epidermal growth factor (EGF) AOD values were0.286±0.034,0.214±0.024,0.146±0.015, vascular endothelial growth factor (VEGF) AOD values were0.280±0.016,0.196±0.032,0.132±0.280, compared with the Vaseline group and Blank group have a significant difference (P＜0.5).Conclusion:Fuzhiqing ointment has significant role in promoting healing of skin ulcers in diabetic rats, this first set out its main mechanism for promoting the proliferation of fibroblasts and new capillaries;stimulating the body’s secretion of vascular endothelial cell growth factor and epidermal growth factor and fibroblast growth factor and speed up the formation of granulation tissue.