Association Between The Subclasses Of HDL And Advanced Oxidation Proteinproducts In Metabolic Syndrome

Objective: To investgate the association between the subclasses of high densitylipoprotein （HDL） and advanced oxidation proteinproducts （AOPPs） in metabolicsyndrome （MS） patients.Method: Plasma concentrations of AOPPs was assayed in352metabolic syndrome（MS） and187healthy subjects by methods of UV spectrophotometry, theconcentrations of lecithin cholesterol acyltransferase （LCAT） and cholesteryl estertransfer protein （CETP） in plasma were measured by ELISA Kits, the subclasses ofplasma HDL were determined by two-dimensional gel electrophoresis conjunctionwith immunodetection method, concentrations of lipids and apolipoproteins in plasmawere detected by automated biochemical analyzer.Results:1. Compared with control, the levels of plasma total cholesterol （TC）, triglyceride（TG）, low density lipoprotein cholesterol （LDL-C）, apolipoprotein B₁₀₀(apoB₁₀₀),preβ₁-HDL, HDL3b, apoB₁₀₀/A1and LDL-C/HDL-C were significantly increasedin MS subjects （P <0.001）, while plasma concentrations of high densitylipoprotein cholesterol （HDL-C）, apolipoprotein A1（apoA1）, LCAT, HDL2bandHDL_2awere significantly decreased in MS subjects （P <0.001）.2. With the increasing of plasma AOPPs levels in MS subjects, the concentrations ofTG, TC, LDL-C, apoB₁₀₀, LDL-C/HDL-C and apoB₁₀₀/apoA1have a rising trend,and the concentrations of HDL-C, LCAT and apoA1have a lower trend.3. The concentrations of preβ₁-HDL, HDL3aand HDL3bhave a rising trend, while theconcentrations of HDL_2ahas a lower trend, with the increasing of plasma AOPPs levels in MS subjects.Conclusion: Concentrations of plasma AOPPs are significantly elevated and HDLsubclasses distribute abnormal in MS subjects, elevated concentrations of plasmaAOPPs related to abnormal HDL metabolism of mature.