The Variation Of Hepatic TMPRSS6，TWSG1and Duodenum Iorn Absorption In Rats After The Acute Exhaustive Exercise

Sports need to consume oxygen, energy and other substances， including in theprocess of the body movement of oxygen transport and utilization needs iron, themain store form of iron as hemoglobin and myoglobin in the body. Early in theexperimental studies have shown that prolonged strenuous exercise can causeexercise-induced hypochrosis, and exercise have important effects on the body of ironhomeostasis., From now on have not reported about acute exhaustive exercise, Howto regulate iron metabolism, and how regulation is restored after exercise. The designof experiment rats acute exhaustion exercise, through the study of the regulation ofiron metabolism pathway in order to clarify the acute exercise on iron metabolismregulation mechanism.Content and Methods:30body weight (220±10) g male SD rats were divided into two group, onecontrol group (CG group,6) and the other exercise group (divided E0h group, E3hgroup, E6h group, E24h group,24). Adapt to exercise before the normal exercise,the adapt stage adapt running speed5m/min, time was30min/d, the slope is0°.Daily ascending order5m/min, until the sixty days to reach treadmill speed30m/min,All to adapt stage6days. After the exercise adapt exercise groups running speed of30m/min in the acute exhaustive exercise until exhaustion. After the end of the exercisegroup were in0h,3h,6h,24h drawn. bDA was detected using nonheme iron induodenum; using Western Blot assay liver STAT3, P-STAT3, duodenal DMT1+,DMT1-,FPN1; using RT-PCR method to detect liver TMPRSS6, BMP6, HJV,SMAD4, the expression TWSG1.Results:1Nonheme iron duodenum after acute exhaustive exercise:Duodenal recovery phase after exercise were significantly higher nonheme ironin E0h, significantly higher than the CG (P <0.01) levels;E3h significantly higher than CG(P <0.01), E3h with no significant differences with E0h, E6h and E24h comparedwith CG no significant change, compared with E0h and E3h significantly decreased(P <0.01).2Detection of liver iron transporter protein after exhaustive exerciseSTAT3: E0h compared with the CG has significantly increased (P <0.01), E3hcompared with the CG group was significantly lower (P <0.01), compared with E0hno significant difference, E6h and CG compared to no significant changes comparedwith E0h and E3h group significantly decreased (P <0.01), E24compared with CGgroup no significant change,and compared with E0h, E3h and E6h significantlydecreased (P <0.05).3Duodenum Ferroportin results After the acute exhaustive exercise:DMT1+: E3h compared with the CG has increased significantly (P <0.01),compared with the same E0h there was a significant increase (P <0.01); E6hcompared with the CG has increased significantly (P <0.01), compared with E3hsignificantly decreased (P <0.01); E24h compared with E3were significantlydecreased (P <0.01), no significant change compared with CG.DMT1-: E0h compared with CG significantly increased (P <0.05); E3hcompared with the CG group significantly increased (P <0.01), compared with E0hsignificantly increased (P <0.05); E6h compared with E3h significantly decreased (P<0.05), compared with the CG no significant difference (P <0.01); E24compared withE3h significantly decreased, there was no significant difference compared with theCG.FPN1: E0h compared with CG significantly decreased (P <0.05);E3h comparedwith the CG were significantly decreased (P <0.01), compared with E0h no significantchange; E6h compared with E3h have increased significantly (P <0.05), comparedwith CG no significant change; E24h compared with E0h and E3h increasedsignificantly (P <0.05), no significant change compared with CG..4Liver iron regulatory genes related result in rats after the acute exhaustiveexercise TWSG1mRNA: E3h expression level was significantly lower than CG (P <0.01),compared with E0h significantly decreased (P <0.05); E6h significantly higher thanE3h (P <0.01); E24h significantly higher than other exercise group (P <0.01). BMP6mRNA: E0h compared with CG significantly increased (P <0.05); E3h significantlyhigher than the other groups (P <0.01). SMAD4mRNA: E3h significantly higher thanthe CG (P <0.01), and compared with E0h significant increased (P <0.05), E6hsignificant lower than E3h, there was no significant difference between CG and E0h,expression of E24h of except E3h each group (P <0.01). TMPRSS6mRNA: E0hcompared with the CG, the expression was significantly decreased (P <0.01)， E3hexpression level of significantly lower than the other group, E24h compared with CGno significant differences, significant higher than E3h and E6h (P <0.01).HJV mRNA:E0h compared with the CG significantly increased (P <0.01), E3h significantly higherthan the other groups (P <0.5)(P <0.01), E24h with CG no difference, less than E0h(P <0.05).Conclusions:（1） After the acute exhaustive exercise, the ability to enhance the intestinalabsorption of iron, but FPN1factor controlling the expression of iron release isinhibited, the use of nonheme iron increased significantly. With the prolongedrecovery time, control the expression of factor increased FPN1iron release, ironreleased from the intestinal, intestinal nonheme iron levels return to normal.（2） After an acute exhaustive exercise, control iron release inhibiting factorFPN1by Hepcidin, its upstream a negative regulator of two factors TWSG1andTMPRSS6on Hepcidin regulation plays a major role, in the recovery process with theand changes in its upstream regulation.（3）The exhaustive exercise induced inflammation can also IL-6-SATA3effecton the regulation of iron metabolism play a role.