| Objective: Intervention by the movement of vascular dementia model rats, the study ofthe brain tissue of vascular dementia nerve growth factor (NGF) expression, superoxidedismutase (SOD), malondialdehyde (MDA) and biochemical indicators of changes inbehavior changes, further confirmed the close relationship between exercise and brain health.Vascular dementia using exercise approach to rehabilitation, prevention of brain damage,delaying brain aging, improve learning and memory ability to provide experimental basis.Methods: Comprehensive literature research advances understanding of exercise andvascular dementia; present study2VO modeling bilateral carotid artery ligation model ofvascular dementia rats, rats were observed behavioral changes in rat brain tissue NGF, proteinconcentration, SOD, MDA and biochemical indicators of change, the impact on vasculardementia learning and memory ability of rats to explore exercise intervention. In this study,2-month-old male pure clean grade Sprague-Dawley (SD) rats were50,randomly divided intothree groups, sham-operated10natural recovery group20, exercise intervention group20.The experiment lasted for eight weeks, sham feeding normal and natural recovery group aftermodeling, treadmill exercise intervention group exercise every day, the first week of4m/min,the second week of8m/min, and the third week later to12m/min. Three groups of rats everyMonday eight-arm radial maze learning and memory tests and weighed. Eight weeks after theunification sacrificed for immunohistochemistry to detect the number of CA1region NGFimmunoreactive cells, hair colorimetric detection of protein in rat brain tissue concentrationsof superoxide dismutase (SOD) activity, malondialdehyde (MDA).Results:1behavioral change: Rats in the eight-arm maze learning and memory test, the short-termlearning and memory, spontaneous recovery group and exercise intervention group wassignificantly higher than the reference memory errors sham group, compared with the very significant difference (P＜0.01); natural recovery group and exercise intervention groupcompared to the fourth week of the second attentive highly significant difference (P＜0.01),spontaneous recovery is less than the error group exercise intervention group; fifth thoughtfulsixth week of natural recovery There were no significant differences (P＜0.05) and exerciseintervention group, but the seventh thoughtful eighth week, the natural recovery group andexercise intervention group with a significant difference (P＜0.01). Long learning andmemory abilities, natural recovery group and exercise intervention group compared with thesham group with a very significant difference (P＜0.01); no significant difference (p firstweek exercise intervention group and spontaneous recovery group（P＜0.05); second therewas a significant difference (P＜0.01) weeks; no significant difference (P＜0.05) sixth weekfifth thoughtful; third week of significant differences between the two groups (P＜0.05)seventh with a very thoughtful eighth week significant difference (P＜0.01).2Biochemical Indexes: Compared with the sham group, natural recovery hippocampal CA1NGF of lnatural recovery group were significantly reduced the number and size ofimmunoreactive cells (P＜0.01, P＜0.01), brain tissue protein concentration and high SODactivity natural recovery group (P＜0.01), MDA content of less than natural recovery group(P＜0.01); sham group and exercise intervention group, NGF immunoreactive cell numberand size of rat hippocampal CA1region were significantly increased (P＜0.01, P＜0.01),protein concentration and SOD activity in the brain tissue was significantly lower thanexercise intervention group (P＜0.01), MDA content than exercise intervention group (P＜0.01); natural recovery group and exercise intervention group compared, NGF exerciseintervention group were immunoreactive cell number and size of the hippocampal CA1regionwere significantly increased (P＜0.05, P＜0.05), brain tissue protein concentration decreased,SOD activity was significantly lower than the exercise intervention group (P＜0.01), MDAcontent than exercise intervention group (P＜0.01).Conclusion:1. The total permanent bilateral carotid artery ligation using the method can effectivelysimulate the function of vascular dementia damage; 2. After eight weeks of exercise intervention, learning and memory impairment and spatialmemory of vascular dementia caused by a significant improvement;3. Appropriate exercise training can increase the protein concentration and activity of SODin brain tissue, inhibiting the growth of MDA content in brain tissue;4. The expression of vascular dementia in hippocampal CA1region of NGF has a closerelationship with brain damage, NGF can effectively protect the nerve cells in the brain,aerobic exercise can significantly increase the expression level of NGF;... |
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