The Development Of New Chemiluminescence Methods And Its Application

Chemiluminescence as an analytical technique is widely applied in toxicological analysis, bioanalysis, clinical analysis and environmental analysis, due to its rapid detection, easy and simple to handle, wide dynamic range, high sensitivity and simple instrumentation. Among numerous reagent can produce chemiluminescence Luminol is one which is most widely used and most in-depth researched. There are many kinds of chemiluminescence reaction system about luminol has been reported. In this paper, we mainly introduced luminol chemiluminescence reaction system in detection of against porcine parvovirus antibodies and hydrogen peroxide.1. Based on gold nanoparticles chemiluminescence immunoassay for rapid and sensitive detection of antibody against porcine parvovirusPorcine parvovirus (PPV) is a small DNA virus of the parvovirus genus, and can be often found among swines throughout the world. PPV is a major cause of infectious reproductive failure in non-immune pigs. In this paper, a rapid, simple, facile, sensitive, and enzyme-amplified chemiluminescence immunoassay method to detect antibodies against porcine parvovirus was presented. The horseradish peroxidase and the detection antibody (rabbit anti-pig IgG) were simultaneously coimmobilized on the surface of gold nanoparticles by the electrostatic method to form gold nanoparticles-based nanoprobes. In optimal conditions, the obtained calibration plot for the standard positive serum was approximately linear within the dilution range of 1:80 to 1:5120. The limit of detection for the assay was 1:10240, which is much lower than that typically achieved by enzyme-linked immunosorbent assay (1:160) (S/N=3). A series of repeatability measurements by using 1:320-fold diluted standard positive serum gave reproducible results with a relative standard deviation of 4.9%(n=11). The ability of the immunosensor to analyze clinical samples was tested on porcine sera. The immunosensor had an efficiency of 90%, a sensitivity of 93.3%, and a specificity of 87.5% relative to the enzyme-linked immunosorbent assay results. The high throughput, sensitivity, selectivity, and reproducibility of our proposed method indicate a promising future for the development of a reliable and ultrasensitive detection of infectious agents and disease markers in clinical analysis.2. Based on bacillus megatherium catalyzing chemiluminescence detection of hydrogen peroxideCell sensor is a detection technology which uses live cells as sensitive elements to make qualitative or quantitative analysis of the detected materials by associating with other detection technology. Hydrogen peroxide is a common material widely existing in natures with both oxidability and reducibility, which is an incompletely reduced metabolite of physiological and biochemical reaction. In this paper, a rapid, simple, easily-operated, sensitive chemiluminescence method, for the detection of hydrogen peroxide has been presented. In optimal conditions, the concentration of hydrogen peroxide can be detected in the range from 5×10-9 mol/L to 1×00-6 mol/L, and the limit of detection was 1×10-9 mol/L. Compared with traditional detection method, it’s greatly simplified the operation process, reduced the cost, broadened the liner range, enhanced the sensitivity. And detection system was very simple, there will be a very strong light signals without the strengthening. The light-emitting time was extended and facilitated detecting.