Effect Of Feeding Different Molecular Weight Condensed Tannin Of The Yanbian Cattle’s Blood Biochemical And Protein Digestive Absorption

Ruminant protein utilization ratio is very low in the process of digestion, most of the feed protein degradation in the rumen fermentation, thus decreasing rumen undegradable protein content, reduce digestion absorption and utilization in the small intestine, and most of the nitrogen emissions, increase the pollution of the environment, waste protein feed resources. In order to prevent protein loss, causing an adverse impact on the topic use rumen protection feed additives-condensed tannins for ruminant digestion and metabolism in vivo experiments. Condensed tannins with strong tannins protein binding ability, functioning rumen undegradable protein, wherein its differences in the molecular weight are absorption content becomes different. Improving animal product’s quality and productivity, also development pollution free natural green feed additives is many scholars of the important subjects. This research provided theory reference.The experiment selected three Yanbian yellow cattle’s have to healthy and the same age and weight. The experiment was in a3×3Latin Square design.Randomly divided into groups of three means control group (group A), large molecular weight tannins group (group B), small molecular weight tannins group (group C). Group A was the control group fed on basal diet, group B add2%large molecular weight condensed tannins among basal diet, and group C add2%small molecular weight condensed tannins among basal diet. Blood biochemical indexes, pH, Feed Intake, Small intestinal nutrient digestibility, Small intestinal nitrogen use efficiency, Amino acid flow were determined in the experiment.Feeding different molecular weight condensed tannins on ruminant digestion and absorption were analyzed.①Small intestine pH over whole time period of showing acidic. Ingest an hour later pH gradual increase, just three hours pH peaked, and then slowly return to the original state. Compared whit group A was higher add condensed tannins test group’s pH than the control group, among the test group, group B>group C, and still relatively stable.②The nutrients intake in the analysis, compared with group A and add different molecular weight of condensed tannins to nutrients intake have no significant difference. This shows the size of molecular weight have nothing to do with nutrients intake content, but according to processing result could for the highest group B.③The dietary nutrient digestion in the analysis, group B dramatically reducing the DM digestibility. This illustrates the bigger molecular weight, the less DM digestibility; CP digestibility, the test group compared with control group significantly lower, between the test group B fall by5.6%(P<0.05); NDF and ADF digestibility are no significant difference (P>0.05) among the three groups.④The small nitrogen utilization in the analysis, As the molecular weights increase, so do the protein absorptive, thereby increasing small intestine protein deposit rate. On the contrary, dramatically reducing fecal nitrogen and urinary nitrogen discharge rate. The show that different molecular weight of condensed tannins can be improved experimental cow’s protein utilization rate.⑤In small intestine to measure flow of amino acid analysis, add the condensed tannins significantly increases its Met, Ala, Glu, Pro, Lys flux(P<0.0.5), but addition to Glu, between the influence of molecular weight-weren’t significant(p>0.05), just group B Glu flow was higher than in the other groups(p<0.05).⑥Feeding different molecular weight of condensed tannins to analyzed blood metabolism condition. Improve the molecular weight of condensed tannins will be increasing serum enzyme and serum protein content. ALT content with group A compared significant difference (P<0.05), group B content the highest; TP, ALB and GLO content compared between groups the more molecular weight, the more on the rise, among the ALB content, control group (A) compared with group B significant difference(P<0.05), and with group C is not significant(P>0.05); ALP activity is test group and the control group(A) remarkable difference(P<0.05), but various molecular weight between is not significant(P>0.05); BUN content as the molecular weight for raising, showed a downward trend(P<0.05); The rest of the questions have change(P<0.05), but among the groups is not significant difference(P>0.05).