| Aralia echinocaulis，which is a type of Araliaceae medicinal plants of genus aralia. Inrecent years, there have been many research on the pharmacology and pharmacodynamicsof Aralia echinocaulis, while their breeding areas has been blank. The same genus plant, A.chinensis, A. elata and other reproductive technoogy have been reported. This study wasdesigned to explore the root rapid propagation techniques, which will have importantpractical significance of plant resources development to Aralia echinocaulis.The results are as follows:(1)Through the cutting experiment of A. echinocaulis, we find that within the testrange, the effects of root length, kinds of rooting regulators, concentration of rootingregulators, rooting regulators soaking time and diameter of cuttings of A. echinocaulis rootsegment cuttage survival rate are significant. But for survival, growth index is notsignificant. Comprehensive analysis, root length in15cm, ABT rooting powderconcentration was100mg·L－1, the cutting’s soaking time is2h, which will be the mosteffective.(2)Tissue culture: the different explants lead to the different response to callusinduction. Usually sterile material need lower concentration of cytokinin and auxin, theadult plants needa longer time and higher levels of concentration of cytokinin and auxin. Asterile blade in A6(MS+6-BA1.0mg·L－1+NAA1.0mg·L－1+2,4-D0.1mg·L－1+AC0.1g·L－1) medium, the induction rate reached100%. Sterile material stems and the three year oldplant the stem explants were A1(MS+6-BA0.5mg·L－1+NAA0.1mg·L－1+2,4-D0.1mg·L－1+AC0.1g·L－1) in the medium, the induction rate was the highest can reach98.33%and96.67%respectively; the three year old plant leaf explants in A4(MS+6-BA1.0mg·L－1+NAA0.1mg·L－1+2,4-D0.5mg·L－1+AC0.1g·L－1) medium induction rate was thehighest, up to96.67%.The callus cultured in growth state six weeks and nine weeks callus had a largerdifference in proliferation and differentiation, differentiation rate of callus was significant(P <0.05), based on Somatic Embryogenesis from cultured C5, C8, C14, C16, where C16with bud. C8medium regeneration appeared plant vitrification, C6had the high rate ofabnormal seedling. Under4levels of PVPP, browning rate levels are similar, the differencewas not significant (P>0.05). The comprehensive analysis showed that the optimal medium, induced callus formation and differentiation of embryogenic callus wasC1(MS+6-BA0.1mg·L－1+NAA0.1mg·L－1+2,4-D0.01mg·L－1+PVPP0.1g·L－1),followed by C6(MS+6-BA0.2mg·L－1+NAA0.2mg·L－1+2,4-D0.01mg·L－1+PVPP1g·L－1)、C14(MS+6-BA1mg·L－1+NAA0.2mg·L－1+2,4-D0.1mg·L－1+PVPP0.1g·L－1)、C16(MS+6-BA1mg·L－1+NAA1mg·L－1+2,4-D0.01mg·L－1+PVPP0.1g·L－1).(3) Research of somatic embryos: the best medium for embryogenic callus wasMS+6-BA0.2mg·L－1+NAA0.5mg·L－1+2,4-D0.5mg·L－1. Somatic embryo induction andthe best cultivation of mature medium without any plant hormones MS and MS+PEG10mg·L－1culture medium. Add PEG10, PEG20can significantly promote the germinationand Aralia echinocaulis somatic embryo maturation, and can reduce the frequency ofabnormal embryos, and when PEG concentration was over50mg·L－1, somatic embryoaccess appear waterlogging phenomenon after three weeks, reducing the effective numberof germination of somatic embryos. The cotyledon embryo white into MS basic culturemedium of7d began to germination,the height growed more than2cm duiring20d.Somatic embryo seedlings without any growth regulators and the addition of0.3mg·L－1NAA medium for culture, were all observed the occurrence of secondary somaticembryo seedling and root tiller. |
PDF Full Text Request