| Sheep breeding character and weight properties is the main economic characters,the attention of researchers both at home and abroad. In recent years, the rise ofmolecular biology technology, make the sheep reproductive performance and weightresearch reached the molecular level, the performance of a certain achievements.From the perspective of genetics, breeding sheep belong to low heritability traits,heritability of0.125, by getting slower genetic progress of traditional selectionmethods, and the effect is not good, and by marker assisted selection for earlyselection, can solve this problem, Because the reproductive traits for quantitative traits,traits and weight may be minor gene control, also may get a few of main gene control,and the impact of environmental effect and the continuous variation. Now on thebehavior of sheep breeding research mainly is the main effect genes as the researchobject, by means of main gene screening and marker assisted selection,implementation in the short term to achieve breeding objectives.Therefore this study from new construction corps, division,165, the Germanmerino sheep,(12) only with Chinese merino ewes (600), selected from the F15RAMS and ewes in300, a total cumulative had record traits of F2generation of150only group.Reproductive character two properties, birth weight and the lambingNumbers.Five weight traits phenotypic traits, birth weight and age of3,6,9monthsweight and body weight. Choose from18chromosome polymorphism is good, allelenumber of9microsatellite marker, correlation analysis was carried out on thereproductive traits and body weight traits, and the chromosome18genetic linkagemapping, QTL mapping for traits difference significantly. The research content andresults are as follows:(1) Good for gome, a slave male sheep for a male parent Chinese merino as femaleparent, building the experimental group150F2generation, and comparing with the Pgeneration, the P and F2generation in lambing number and3months there weresignificant differences on the weight.Shows the two properties in offspring in theseparation, this group can be used in the construction of linkage map and QTLmapping.(2) In the experimental group because some individuals build relationship not sure,so do to the paternity test.This experiment adopts the method of microsatellite DNAmolecular markers,132through the paternal half-siblings Chinese merino sheep inBM4621, BM143, OarHH55, OarJMP8, BM6438, BM6506, BM1824, OarDB6,ILSTS0049microsatellite loci analysis, calculated the Chinese merino sheep in thenine sites allele frequency and its distribution rule, the nine microsatellite loci arecharacterized by high or moderate polymorphism, and the seven groups ofparent-child paternity tests.Results show that the nine microsatellite loci in test toselect7groups of parents and children, the probability of99.98%~99.99%. Improvethe group’s pedigree data.The construction of linkage map and QTL mapping laid afoundation.(3) based on DNA extraction, PCR and electrophoresis, of phenotypic traits andstatistical analysis of genotype, the nine microsatellite in the experimental group of the communist party of China have found47alleles, the average allele was5.22, mostof them at the TGLA122for7, BMS3413, CSSM018OV, CSAP28E for6, BMS1561and MCM148for five, BMS2815, TGLA337and BMS1494for four.Amplification ofalleles length between97~237bp.Based on index of polymorphism informationcontent, highly polymorphic seat for PIC>0.5, moderate polymorphism seat is0.25<PIC <0.5, low polymorphic loci for PIC <0.25. This experimental results: the PICand group high heterozygosity, polymorphism, the allele number.We selected9microsatellite markers, including6highly polymorphism, moderate polymorphismshave3, so for us in the construction of genetic linkage map and QTL mapping laid afoundation.(4) reproductive traits and body weight traits phenotypic value differences Fstatistical test: on reproductive traits, have two tags tight chain BMS2815andTGLA337lambing number exist significantly differencet with the lamb, p <0.05,there may be a between the two tags affect the number of lambing QTL. On weighttraits, also have two tight chain tags BMS1561and CSSM0180V and sheep3monthsof age weight had significant difference (p <0.05, see you in these two tags has apossible influence of3months of age of QTL.(5) the method of using crimap2.4linkage map construction and QTL express.Thechromosome18genetic linkage mapping, as well as to the lambing number3monthsof age and weight for QTL mapping. On the sheep breeding traits, detected in56cMin chromosome18a sheep lambing number of QTL effects.On the weight traits in100cM test generation of a sheep3months weight of QTL. |
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