Sheep Chromosome13Reproductive Traits Relating Analysis Of QTL Mapping

Sheep is one of the earlier human domestication of animals, but because of somecharacteristics of the sheep, make people always can’t carry on the real modernefficient breeding.Livestock breeding value in many economic traits are to measurethe number of characters, such as producing wool and meat, eggs, milk andreproductive performance, and so on, which directly affects the economic benefits oflivestock production.Growth related traits are commonly believed to be controlled by tiny effectpolygene quantitative traits, because the reproductive traits for quantitative traits,traits and weight may be minor gene control, also may get a few of main gene control,and the impact of environmental effect and the continuous variation. Therefore thisstudy from new construction corps in the first division,165, the German merino sheep(12) and the Chinese merino ewes (600), selected from the F1five RAMS and ewes in300, a total cumulative had record traits of F2generation of150only group.Bodymeasurements traits5phenotypic traits, body height, body length, chest width, waistwide Angle, body weight.Reproductive character two properties, birth weight and thelambing Numbers.Selected from13chromosome polymorphism is good, allelenumber of10microsatellite marker, correlation analysis was carried out on the bodymeasurements traits and reproduction traits, and the chromosome13genetic linkagemapping, significant difference for the traits of the QTL mapping.The researchcontent and results are as follows:(1)good for gome, a slave male sheep for a male parent Chinese merino as femaleparent, building the experimental group150F2generation, and comparing with the Pgeneration, the P and F2generation in adult weight and there were significantdifferences on the lamb birth weight.Shows the two properties in offspring in theseparation, this group can be used in the construction of linkage map and QTLmapping.based on DNA extraction, PCR and electrophoresis, of phenotypic traits andstatistical analysis of genotype, this research selected10microsatellite50alleles havebeen found in experimental group of the communist party of China, the average allelewas five, most of them at the SCYAMS, BL1071, for7, BMS1669fortwo.Amplification of the length of alleles between109~326bp, this experimentresearch results as follows:10tag selected microsatellite markers have four presenthigh polymorphism,6moderate polymorphism, for sheep chromosome13geneticlinkage map construction and QTL mapping provides a guarantee.(2) the differences in reproductive traits and body weight traits phenotypic value ofF statistical test: in the line of built by reference in this experiment, the sheepchromosome13microsatellite marker TGLA6and tag BMC1222detected may existbetween sheep breeding traits of QTL loci affecting sheep birth weight, location onchromosome1315.3cM, by F test has reached the significant level (P <0.05), about4.8cM from BMC1222;In sheep chromosome13microsatellite marker MCMA2andtag MCM253detected may exist between the sheep in the body measurements traitsaffect sheep QTL loci of adult weight, location on chromosome13to74cM, the Ftest has reached the significant level (P <0.05), is about3.5cM MCM253. (3) the method of using crimap2.4linkage map construction andGRIDQTL.Mapped the chromosome of genetic linkage map,13of birth weight andadult weight and QTL mapping.In sheep breeding characters, on chromosome1315.3cM, detect an impact sheep birth weight of QTL.In building a group of people is onthe body measurements traits of a QTL influence adult sheep weight in74cM.