The Immunomodulatory Function And Safety Evaluation Of The High-purity Chinese Compound Polysaccharide

Chinese herbal drugs are rarity of traditional medicine, which effective constituents are polysaccharide. It has been confirmed that the Polysaccharides from Chinese herbal drugs have immunological activity, antitumor activity, anti-inflammatory, antiviral activity, anti-radiation effect et.al. Immunity regulate function is the focus of polysaccharides research. At Present Stage, the single medical material is widely studied, not compound Chinese medicinal herb polysaccharide. The compound Chinese medicinal herb rather than the single medical material was used to treat diseases in traditional China. The best dose effect relationship in compound Chinese medicinal herb is better than the single medical.Aim:This study explore immune enhancement mechanism of polysaccharide, which exact from the Chinese Herbal Medicine, has been purified by LiZanyang. Thread detect levels of ND antibody titer, lymphocyte transformation rate, Superoxide Dismutase(SOD), erythrocyte rosette rate, Security and Stability Analysis, discuss relation the optimum purity Chinese medicinal herb compound polysaccharide with acute in syndrome.Methods:At8-14days of chicks, three doses of high purity polysaccharide was given for7days, blood sampling and separate serum. The ND antibody titer, lymphocyte transformation rate, Superoxide Dismutase (SOD) and erythrocyte rosette rate was be detected to find the optimum dose.(2) Oral and injectable of different gradients were deliveried to mice, to test its acute toxicity. The guinea pigs were intraperitoneal injection test its systemic allergic. Using hemolytic test in vitro, the effect of hemolytic test was observed. The heat source and muscle irritation were test by use intravenously and intramuscular injection.(3) The OD value was test with different temperature, light, oxidizing agents, reducing agents, pH and additives for the right preservation condition.Results:(1) Three concentrations of compound polysaccharide all can raise the ND antibody titer, lymphocyte transformation rate, E-rosettes rate and SOD activity. And the Chinese herbal medicine compound polysaccharide is better than Astragalus polysaccharide and levamisole groups. The middle dose group (25mg/mL) is better than other dose groups of the optimum purity Chinese medicinal herb compound polysaccharide.(2)The optimum purity Chinese medicinal herb compound polysaccharide Injection had no allergy effect and hemolysis phenomenon, it had no siginificant stimulation effect on muscles and vessel. So the experiments provide a theoretical basis for clinical application of the optimum purity Chinese medicinal herb compound polysaccharide Injection.(3)The optimum purity Chinese medicinal herb compound polysaccharide was good in low temperature, with reductants and pH of5.0-7.0. However, when oxidizers(H2O2), citric acid, sodium benzoate, etc were exist, the stability was poor.Conclusion:The purity of compound polysaccharide has a good role in immune regulation, especially middle dose (25mg/ml). The optimum purity Chinese medicinal herb compound polysaccharide Injection had no allergy effect and hemolysis phenomenon, it had no siginificant stimulation effect on muscles and vessel. So the experiments provide a theoretical basis for clinical application of the optimum purity Chinese medicinal herb compound polysaccharide Injection. The optimum purity Chinese medicinal herb compound polysaccharide was good in low temperature, with reductants and pH of5.0-7.0. However, when oxidizers(H2O2), citric acid, sodium benzoate, etc were exist, the stability was poor.