Research On Predominant Chemical Compositions And Antioxidative Activities Of Pleurotus Ostreatus Fruiting Bodies

In our study, we cultivated mushroom by 80% formula cottonseed hull, corncob 10%, 1% lime, sugar 1% carbendazim, carbendazim 1%, wheat bran 8%; solid-liquid ratio to determine the best training materials for 1: 1.25, hyphae in the first 10 days after inoculation sackful, mushroom fruiting bag full days to 30 days, the full number of days required fruiting bag to eight days, mushroom biological efficiency average of 83.7%, the mean water content to 90.25%.In this study, a mature mushroom fruiting separation of active ingredients, with 95% ethanol extract 5.2 kg of dry mushroom fruiting bodies, the solution was concentrated after extraction, concentrated solution of ethyl acetate and n-butanol, respectively extract was concentrated and dried get the ethyl acetate extract 32 g, n-butanol extract 26 g, Butanol extract by silica gel column chromatography to obtain three compounds, by classical chemical methods and a variety of advanced spectroscopic techniques(including 1HNMR, 13CNMR) identified compound uracil riboside total 286 mg, compound 2(1’R, 2’R, 3’R, 4’R) 1,2,4- triazole nucleoside, a total of 235 mg, the compound 3 is mannitol co 187 mg, ethyl acetate extract was purified by silica gel column chromatography three compounds were obtained compound 4 is n-hexadecanoic acid co 93 mg, compound 5 is ergosterol co 82 mg, compound 6 is n-pentadecane acid co 65 mg.In this study, the ethyl acetate extract of mushroom fruiting bodies and their study of aging caused D-galactose induced n-butanol extract, to explore its antioxidant mechanisms in vivo. The results show that both the ethyl acetate extract and n-butanol extract can improve the mice suffered organ damage, reduce the quality of the liver, spleen and brain to improve quality, mushroom fruiting body extract can improve because of D- galactose-induced Aging mouse serum SOD and GHS-Px, reduced MDA content, while each treatment group can significantly improve the mouse heart, brain and liver SOD, MDA and GSH-Px levels, indicating a certain mushroom extracts in vivo antioxidant activity, the mechanism may be related to the mushroom extract can remove the body of excess free radicals.