The Impacts Of Shao’s Method Of Wu Zhen On The Expression Of CGRP And NGF In Asthma Model Rats

Objective:This study is based Chinesemedicine understanding of bronchial asthma, put forward Shao’s Method of Wu Zhen is "FengShu (double) ", "DaZhui", "FengMen (double) "treatment of bronchial asthma model in rats, Level of CGRP and NGF plays expression in the lung tissue was observed and the influence of HE staining lung tissue pathology, to study the effect of acupuncture treatment of asthma pathogenesis, provide scientific theory basis for acupuncture treatment of bronchial asthma.Method:40 male SD rats were randomly divided into four groups:First group-blank control group, second group-model control group, third group-drug treatment group, fourthly group-acupuncture treatment group, and each group of ten. In addition to the blank control group of ten, Other 30 adopt intraperitoneal injection of egg albumin (OVA) with 1% OVA sensitization to solution atomization inhalation trigger asthma rat model was established. Blank control group was in the same time giving physiological saline. After the excitation model control group, did not do any other processing; The drug treatment group on intraperitoneal injection of dexamethasone treatment after each excitation; After acupuncture treatment group at each time stimulate acupuncture "Feng Shu"(double), "Da Zhui", "Feng Men"(double) treatment; Line after the treatment, HE staining and immunohistochemical method in the determination of the lung tissue of calcitonin gene related peptide (CGRP), nerve growth factor (NGF) plays expression, observation rat airway morphology change and CGRP and NGF plays in asthma model the expression of lung tissue in rats.Results:1. Each group behavioral signs observed in ratsBlank control group:breathing smooth, action quick and normality. Model control group:sneezing, shortness of breath, unresponsive, a few serious lying motionless in rats. The acupuncture and drug treatment group:sneezing, shortness of breath and other symptoms than the model control group significantly reduce, reaction is more sensitive.2. Each group compared with rat lung tissue morphologyBlank control group:the rat bronchial lung tissue structure basic integrity. Basic normal lung tissue morphological structure. The model control group:rats bronchial mucosa epithelial damage, airway wall thickening, a large number of inflammatory cells infiltration, lung tissue alveolar structure disorder. The acupuncture treatment group and drug treatment group:the airway epithelium with occasional loss, bronchial structure is relatively intact, lung tissue inflammation infiltration cells less, alveolar structure essentially normal.3. Each group compared with model CGRP lung tissue in ratsBlank control group:Occasionally seen in the rat lung tissue of tan dyeing CGRP positive cell cytoplasm. Model control group compared with the blank control group, the lung tissue of CGRP positive quantity increased obviously, the difference is statistically significant(P<0.01); Acupuncture treatment group, drug treatment group compared with the blank control group, higher positive expression of CGRP, statistically significant difference(P<0.01); Acupuncture treatment group, drug treatment group compared with model control group, the positive quantity were significantly reduced, statistically significant difference(P<0.01). Acupuncture treatment group compared with the drug therapy group, the difference is statistically significant(P<0.01)4. Each group compared with model NGF lung tissue of ratsBlank control group:occasionally seen in the rat lung tissue of rats tan dyeing NGF positive cell cytoplasm. Model control group compared with the blank control group, model NGF positive expression in lung tissue increased obviously, statistically significant difference (P<0.01); Acupuncture treatment group, drug treatment group compared with the blank control group, higher positive expression of NGF plays, statistically significant difference (P<0.01); Acupuncture treatment group, drug treatment group compared with model control group, the positive quantity were significantly lower, statistically significant difference (P<0.01). Acupuncture treatment group compared with the drug therapy group, the difference is statistically significant (P<0.01)Conclusion:1. Shao’s Method of Wu Zhen can improve the signs and symptoms of asthma model rats.2. Shao’s Method of Wu Zhen can reduce asthma model in rat lung tissue inflammatory cells, and inhibit the release of inflammatory mediators, repair the disorder bronchial mucosa structure.3. Shao’s Method of Wu Zhen can make the asthma model rats obviously decreased expression of CGRP and NGF plays in the lung tissue. That acupuncture reduce positive expression of CGRP and NGF plays is one way for the treatment of asthma.