| Colonic carcinoma is a common gastrointestinal cancer. In recent years, with the continuous enhancement of the people living standards, changes in eating habits and structure, and ulcerative colitis, a history of intestinal polyps, an aging population and environmental factors, etc., our colonic carcinoma incidence and mortality rates are upward trend. colonic carcinoma etiology, clinical presentation, the molecular mechanisms of cancer and many other obvious differences, In the clinical treatment of colonic carcinoma is found, the same stage and pathological types of patients, with the same regimen,but the efficacy and prognosis have significantly different. So exploring the molecular mechanisms of cancer formation, searching the markers of canceration process have important clinical significance of colonic carcinoma prevention and treatment.α-tubulin(α-tubulin) is an important component of cell organelles central body, involved in mediating microtubule continuous extension in conjunction with dynein,in mitosis of tumor cells have an important impact, mainly involved in the maintenance of cell shape, chromosome separation and other process. When the tumor cell proliferation active,and mitosis is active, causing a corresponding abnormal expression of α-tubulin, thus becoming an important factor in the process of tumor development。STK15(ser-ine / threoninekinase 15 gene,) is a cancer gene, which encodes a centrosome-associated kinase(serine / threonine kinase), plays an important role in the mitosis process, involved in regulating centrosome maturation and microscopic crystals nucleus formation, generating and maintaining a bipolar spindle and in the equal distribution of chromosomes plays in a significant role, STK15 abnormal amplification and overexpression can induce abnormal changes in the number of the center 、chromosomal cells, instability, the formation aneuploidy in cells and malignant transformation of leading to tumorigenesis.p53 is the most common mutations gene in human cancer, in the changes of the cell cycle are also located in the Centrosome, plays an important role in the replication and regulation of function of Centrosome. Inactivation and mutations of p53 can enables centrosome duplication occurs repeatedly in round cell replication cycle, resulting in doubling the number of chromosomes and promote tumor formation. Studies have shown that, p53 can through multiple pathway effects centrosome duplication and separation.Objective:By detecting expression centrosome-associated protein that is α-tubulin, STK15 and p53 in colonic carcinoma, to explore the correlation of colonic carcinoma with the expression and clinicopathological factors and the relevance of the three proteins,can provide a theoretical basis for clinical diagnosis and prognosis of patients with Colonic carcinoma.Methods:Collecting 72 cases colonic carcinoma by surgical excision and pathological diagnosis and 36 cases adjacent normal non-cancerous tissues with pathology wax blocks. By applying immunohis- tochemistry Envision two-step detecting the expression of α-tubulin, STK15 and P53 protein in colonic carcinoma, analysed statistically the correlation among them with clinic-pathological differentiation gender, age, tumor size, tumor differentiation, Prognosis、 TNM stage,Depth of invasion and analyzed the relationship among three。The data were analyzed by using SPSS 17.0 statistical software, count data using c2 test, Correlation using Spearmancorrelation analysis, α=0.05 as tests, with P<0.05 for the difference was statistical significant.Results:1.α-tubulin, STK15 and P53 expression rate in colonic carcinoma tissues was significantly higher than in adjacent normal tissue, α-tubulin, STK15 positive staining was localized in the cytoplasm, P53 protein positive staining localized in the nucleus. In Colonic carcinoma three proteins positive rates were 62.5%(45/72), 65.3%(47/72), 69.4%(50/72). Significantly higher than in adjacent normal tissue 5.6%(2/36), 2.8(1/36), 5.6%(2/36), the difference was statistically significant(c2 = 31.66,37.97,39.24 P less than 0.05).2. The expression of α-tubulin, STK15, P53 in colonic carcinoma tissue were all closely with TNM stage, depth of invasion,(all P <0.05), in which the later stages of tumor,and the higher expression of three proteins in colonic carcinoma, the infiltration deeper level, the higher expression level of α-tubulin, STK15, P53 were; while the expression level of α-tubulin, STK15, P53 were not all closely with gender, age, tumor size, differentiation and other clinical factors(P> 0.05).3.The positive expression of STK15 in α-tubulin positive groupwas 81.8%,it was positively rate significantly higher than the expression of STK15 of 24.0% in the negative expression group,α-tubulin and STK15 expression was positively correlated(P<0.05);The positive expression of p53 in STK15 positive group was 87.2%,it was positively rate significantly higher than the expression of p53 of 36.0% in the negative expression group,STK15 and p53 expression was positively correlated(P<0.05); The positive expression of p53 in α-tubulin positive group was 82.2%,it was positively rate significantly higher than the expression of p53 of 48.1% in the negative expression group,α-tubulin and p53 expression was positively correlated(P <0.05);Conclusion:α-tubulin, STK15 and P53 were overexpressed in colonic carcinoma, and the expression were significantly higher than in adjacent normal tissue,uggesting that the three caught involved in the occurrence and development of colonic carcinoma,the expression levels of α-tubulin, STK15 and p53 were not correlation with sex, age, tumor size, degree of differentiation,CloselyCorrelation with TNM stage and depth of invasion of colonic carcinoma, the infiltration deeper level, the higher expression level of α-tubulin, STK15, p53 were, and in clinical stage Ⅲ-Ⅳstage group was significantly higher than the expression level of in linical stage Ⅰ-Ⅱ stage group suggesting, suggesting overexpression of three closely related to colonic carcinoma occurrence and development.α-tubulin and STK15, STK15 and p53, α-tubulin and P53 expression levels have a positive correlation between,tip the three possible existencing of a regulatory relationships in the development together to promote in colonic carcinoma,joint detection α-tubulin, STK15 and p53 have an instructive in judging the potential of metastasis and prognosis in colonic carcinoma. |
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