The Study On Identification And Toxic Components Of Tibetan Medicine Radix Aconiti

Radix aconiti is a commonly used Tibetan medicine. According to the Tibetan medicine ministry records,there are two kinds of Radix aconiti:Aconitum pendulum Busch and Aconitum flavum Hand.-Mazzbut. And Aconitum kongboense Lauener is also a commonly used Radix aconiti. while Aconitum gymnandrum Maxim is not used as Radix aconiti, which has similar external to Aconitum pendulum Busch.The current standard of Radix aconiti quality control only consist of morphological identification and physicochemical identification,is not enough tocomprehensively control the quality of Radix aconiti.In the study, Aconitum pendulum Busch and Aconitum kongboense Lauene were compared from the macro morphology, root tissue structure characteristic and medicinal powder.At first,the TLC conditions of Aconitum pendulum Busch of effective components in aconitine was established, aime to identify Aconitum pendulum Busch and Aconitum kongboense Lauener by TLC quickly.Established a Characteristic spectrum and a quantitative analysis of Aconitum pendulum Busch,using aconitine as index.Revised quality standards of Aconitum pendulum Busch.Compared with Aconitum pendulum Busch, Aconitum kongboense Lauener and Aconitum gymnandrum Maxim by using HPLC method to determine 6 kinds of alkaloids for control the quality of Radix aconiti.The result show that Aconitum pendulum Busch and Aconitum kongboense Lauenethere slightly different in appearance, Aconitum kongboense Lauener is shorter and has more rootlets.In parafin section of root tissue structure,the cambium of Aconitum pendulum Busch is obvious, vessels line in coner of cambium or like "U",the cambium of conitum kongboense Lauenethere is not obvious,vessels extend to the pith.In the medicinal powder, both are off-white color,and have many starches which size and shape were different.TLC analysis revealed that Aconitum pendulum Busch has aconitine,but Aconitum kongboense Lauener do not have.HPLC analysis revealed that components of Aconitum pendulum Busch and Aconitum kongboense Lauene have large difference,aconitine and 3-acetylaconitine in Aconitum pendulum Busch, deoxyaconitine in Aconitum kongboense Lauener,deoxyaconitine in Aconitum gymnandrum Maxim. Establish .Finaliy the Characteristic spectrumthe and the quality standard of Aconitum pendulum Busch was established, the quality of Aconitum pendulum Busch could be controlled.