Impact Of LPL Gene Rs283 Polymorphism On Exercise-induced Changes In Obese Adolescents And The Regulatorv Mechanisms Behind It

Objectives:To explore the association between rs283 polymorphism located in the lipoprotein lipase (LPL) gene and exercise-induced changes in body composition, lipid and glucose metabolism in obese adolescents, and to probe into the molecular regulatory mechanisms.Design and Methods:53 obese adolescents of Han nationality conducted an aerobic training for four weeks. Body composition, lipid and glucose metabolic parameters were tested before and after the training. The rs283 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism and association analysis with weight-reducing effect was performed. The regulatory mechanisms of rs283 polymorphism were explored through duel-luciferase reporter assay.Results:Exercise-induced change rates were as follows:ΔBody Fat(BF)% of GG genotype groups was 3.37±1.60. significantly higher than that of GA genotype groups (2.09±1.53, P<0.01); ΔlgHomeostasis Model Assessment-Insulin Resistance(HOMA-IR) was 0.52±0.13, obviously higher than that of GA genotype groups(0.44±0.10, P<0.05); and ΔTriglyceride (TG) was 51.91±6.56, greatly higher than that of GA genotype groups (47.06±5.36, P<0.01). The relative luciferase activity of the reporter gene in recombinant vector carrying the G allele was 2.67±0.22, markedly higher than that in recombinant vector carrying the A allele (1.63±0.03, P<0.01)Conclusions:Chinese obese adolescents of Han nationality with GG genotype of rs283 polymorphism were more sensitive to exercise-induced weight loss. The G allele can improve reporter gene expression level, indicating the effects of rs283 on gene expression.