The Effects Of Swimming Movement And Rich Environment On Autism Rats’ Learning And Memory Ability And Mechanism Research

Purpose: Swimming movement and rich environment is studied and discussed the influence of model rats learning and memory ability of autism and neurobehavioral effect through the PSD- 95 protein expression level reveals the influence mechanism.Methods:This experiment by means of pregnancy by intraperitoneal injection of 12.5 days of Wistar rats VPA600mg/kg(VPA concentration of 250 mg/ml), reproduce the pups to behavioral detection for autism in rats, and the experiment pups. At the same time pregnant mice celiac injection of physiological saline as normal controls, 21 days weaning. 23 days after their birth experimental intervention, the experiment is divided into five groups: blank control group,control group,swimming group, rich environmental,swimming+rich environmental group.Swimming for 3:00 PM every day- at 4:30 in the 30 + /- 1 ℃ water temperature for 1.5 hours of moderate exercise load training, training of eight weeks. Rich environment for length, width and height of 100 cm * 60 cm x 40 cm transparent raised in a cage, with quiet running wheel, support wheel, crazy play plate, bell swings, seesaw, rock climbing, "daruma" mice, transparent light bouncing ball, tunnels, line ball cage rabbit skin of mice, such as plastic small ring, rubber ball hedgehog, let autism rats in the training. After eight weeks, within three days of hungry mice, and to keep his weight in the free food to about 85% of body weight, each group of mice eight arm maze experiment, testing the working memory error number, number of reference memory errors and finish all the time. Then perfusion fixation, brain, brain slices, dewaxing, by immunofluorescence chemical dyeing method, to detect the hippocampus nerve CA1 area of PSD- 95 protein levels, application of the Image- Pro Plus multi-function true color Image analysis, the cell Image analysis management system for average fluorescence intensity calculation of PSD- 95 protein(fluorescence intensity/total area) and contrastive analysis.Results:(1) eight arm maze test 1. Total feeding time, the first group, the third and fourth group and five group average is less than the second group, P < 0.01, there are differences between groups, statistically significant; The third and the fourth group, the fourth group compared with five group, P > 0.05, no statistically significant; The third group compared with five group, P = 0.066, close to 0.05. 2. Working memory errors, the first group, the third group, the fourth group, and five group average is less than the second group, P < 0.01, there are differences between groups, statistically significant; The third and the fourth group, the fourth group compared with five group, P > 0.05, no statistically significant; The third group compared with five group, P < 0.01, have differences between groups, with statistical significance. 3. The reference memory errors, the first group, the third and fourth group and five group average is less than the second group, P < 0.01, there are differences between groups, statistically significant; The third and the fourth group, the fourth group compared with five group, P > 0.05, no statistically significant; The third group compared with five group, P = 0.067, close to 0.05.(2) immunofluorescence chemical dyeing test In protein average fluorescence intensity on PSD- 95, the first group, the third group, the fourth group, five group were greater than the second group, P < 0.01, the clear differences between groups, statistically significant; The third and the fourth group, the fourth group compared with five group, P > 0.05, no statistical significance; The third group compared with five group, P < 0.01, the clear difference between group, with statistical significanceConclusion:(1) the swimming sports, rich environment, swimming exercise combined with rich environment can significantly improve the learning and memory ability of rats with autism; On three intervention effect comparison, rich environment intervention was slightly better than swimming exercise intervention, intervention was slightly better than swimming in combination with rich environment rich environment intervention, intervention is better than swimming in combination with rich environment swimming exercise intervention.(2) the intervention and swimming swimming exercise intervention, rich environment combined with rich environment intervention method can obviously promote the autism PSD rat hippocampal CA1 area nerve cell- 95 protein expression; On three intervention effect comparison, rich environment intervention was slightly better than swimming exercise intervention, intervention was slightly better than swimming in combination with rich environment rich environment intervention, intervention is better than swimming in combination with rich environment swimming exercise intervention.