Effects Of Shen Ling Jian Pi Wei Granules On Oxidative Stress Reaction In Mice With Non-alcoholic Fatty Liver Disease

Objective:By copying the non-alcoholic fatty liver disease model in mice,to observe the changes on MDA, SOD and CYP2E1 of mice with NAFLD which have treated with Shen ling jian pi wei Granules, and study the possible mechanisms that Shen ling jian pi wei Granules reduce oxidative stress and lipid peroxidation.Methods:1,Model preparation: Gave numbers to 190 KM mice, randomly divided into 5 roups.Nonrmal group (50) were given the ordinary feed, model group (50) and low-dose Shen ling jian pi wei Granules group (30) and middle-dose Shen ling jian pi wei Granules group(30) and high-dose Shen ling jian pi wei Granules group(30) were given the high fat feed.2,Grouping dosing: Gave normal group and model group to saline began from 6 weekend, gave the other groups to Shen ling jian pi wei Granules untill 12th weekend.The low-dose Shen ling jian pi wei Granules group were given a gavage at 1.3g�kg-1 every day, the middle-dose Shen ling jian pi wei Granules group were given a gavage at 2.6g�kg-1 every day,the high-dose Shen ling jian pi wei Granules group were given a gavage at 5.2g�kg-1 every day.3,In 4th, 6th, 8th, 10th, 12th weekends, dynamic observed each group mice liver index,serum liver function (ALT, AST) and blood lipid (serum TC,TG).Liver tissue parafin section with HE staining, dynamic observed the hepatic steatosis.Using colorimetry testing each group mice liver tissue MDA and SOD content,using real time quantitative RT-PCR detection each group mice liver tissue CYP2E1 mRNA expression, using western blotting detection each group mice liver tissue CYP2E1 protein expression,.Results:1,Pathology:The model group mice pathologic shown that a small number of lipid droplets cavity can be seen in the cytoplasm of liver cells from 4th weekend;In 6th weekend the perspective unit area of more than one-third of the hepatocyte degenerati on can be seen under microscope;In 8th weekend the nucleus of some serious fusion of lipid droplets can be squeezed into the cell membrane;In 10th weekend fat liver ce lls has become more widespread, liver cell cytoplasm was loose,even became balloon samples;In 12th weekend liver cells damage more seriously than 10th weekend,a small number of liver cells had undergone karorrhexis and karyolysis.The damage of hepatic ti ssuses of the drug group is dacreased,the middle dose group and the high and middle dose group is better than the low dose group.2,Influence on the liver index, blood lipid(TC,TG),serum liver function(ALT,AST),with Shen ling jian pi wei Granules:The liver index, blood lipid(TC,TG),serum liver f unction(ALT,AST)is increased with the building time and has significant statistical diffe rence(P<0.05);Compared with the model group,these indexes of each dose invigorating spleen recipes group are decreased and have significant statistical difference(P<0.05).3,Influence on MDA and SOD of mice hepatical tissues with Shen ling jian pi w ei Granules:MDA of mice hepatical tissues is increased with the building time and has significant statistical difference(P<0.05),SOD of mice hepatical tissues is decreased wi th the building time and has significant statistical difference(P<0.05);Compared with t he model group, the decrease of MDA and the increase of SOD of each dose invigor ating spleen recipes group has significant statistical difference in 8th?12th(P<0.05).4,Influence on expression of CYP2E1 mRNA and CYP2E1 protein of mice hepati cal tissues with Shen ling jian pi wei Granules:Expression of CYP2E1 mRNA and CY P2E1 protein of mice hepatical tissues is increased with the building time and has sig nificant statistical difference(P<0.05);Compared with the model group,each dose Shen li ng jian pi wei Granules group is decreased and has significant statistical difference in 8th?12th(P<0.05).Conclusion:1,By high fat feed mice, typical liver fatty change appeared in 6th weekend and prompt fatty liver mice model copyed success.2,Shen ling jian pi wei Granules can increase SOD of NAFLD mice hepatical tissues,decrease MDA,expression of CYP2E1 mRNAand CYP2E1 protein of NAFLD mice hepatical tissues.It show that Shen ling jian pi wei Granules improve the injury of liver cells of NAFLD mice may be related to reduce oxidative stress and lipid peroxidation.