| Objective: More and more studies in the field of sports science have shown that appropriate exercise has a positive effect on learning and memory.In this study,through effects of six weeks of aerobic treadmill training on SD rats' ability on learning and memory,the hippocampus and cortex oxidative and antioxidant,to explore different forms of continuous and intermittent exercise promoting learning and memory in rats by changing the metabolism of MDA,SOD and expression of NO to realize.Methods: Screening experimental animals after the adaptive feeding.Screening methods adopt water maze visualization platform experiment,elimination of suspected congenital dementia rats.Selecting 24 healthy male adult SD rats,and randomly divided into blank control group(n = 8),continuous training group(n = 8)and intermittent training group(n = 8).the formal experiment starts before a week adaptation treadmill training.Formal treadmill training adopts in the form of an incremental load,the starting speed is of 22 m / min with a gradient of 0,weekly increments 1m / min for 6 weeks,the last week is of speed of 27 m / min;continuous group sets the movement for 40 min,intermittent group sets movement for 20 min,2 times a day.After treadmill training,three groups of SD rats start a five-day of Morris water maze test(four days of place navigation test,one day of space exploration experiments).Fast the rats for 24 h after the water maze test,inject with 3.6% chloral hydrate intraperitoneal of anesthesia,remove the hippocampus,cortex from the brain.Use the kit to test hippocampus,cortex MDA content,SOD vitality,NO content.Results:(1)Morris water maze test: a longitudinal comparison has found in the navigation test that with increasing training time,the latency in each group has reduced,the distance percentage and time percentage of platform quadrant has increased;a horizontal comparison has found that on the second day the latency between exercise group and control group has a significant difference(p <0.05);on third day,compared with the control group,the continuous group was significantly decreased(P <0.05),the intermittent group decreased significantly(p <0.01),wherein intermittent group compared with the group continued,intermittent group was significantly decreased(p <0.05);on fourth day,the latency period of the exercise group was significantly lower than the control group(p <0.01).From the platform quadrant,the distance percentage of intermittent group is the highest in the four-day test;the third day of intermittent group was significantly higher than the control group,continuous group(p <0.05),the fourth day of intermittent group was significantly higher than the control group(p <0.05).In the four days of testing,of the platform quadrant time percentage,the highest is intermittent group,followed by continuous group and control group;on the third day,the intermittent group,of the platform quadrant time percentage,is higher than the control group that the difference was highly significant(p <0.01);on the fourth day,the intermittent group was significantly higher than the continuous group and the control group that the difference was highly significant(p <0.01).Space Exploration Experimental results show that compared with the control group,the continuous group and the intermittent group number of crossings increased significantly(p <0.01);the continuous group and the intermittent group arrival latency was significantly lower than the control group(p <0.01),between the exercise group found no differences;of platform quadrant distance percentage and platform quadrant time percentage,the intermittent group was significantly higher(p <0.05)than the control group,no difference between the two exercise groups;total distance of the exercise group was significantly higher than the control group(p <0.01),between exercise groups found no difference;average speed on continuous group(p <0.05)and the intermittent group(p <0.01)were significantly higher than the control group,but no difference found between the exercise group.(2)the hippocampus ability of oxidative and anti-oxidation results: compared with the control group,MDA content in the continuous group and the intermittent group(P <0.01)was significantly reduced,the intermittent group lower than the continuous group,but no difference;SOD vitality in continuous group and intermittent group was significantly higher than the control group(P <0.01),the continuous group was higher than intermittent group,but no difference between groups.Compared with the control group,the exercise group with higher levels of NO content,wherein the intermittent group and the control group,there was a significant difference(P <0.05),but no difference between intermittent group and continuous group.(3)cortex ability of oxidative and anti-oxidation results: the MDA content in continuous group and intermittent group was lower than the control group(P <0.05), the intermittent group was lower than the continuous group,but no difference between two groups;SOD vitality in the continuous group and the intermittent group was higher the control group,the intermittent group was higher than continuous group,but there was no difference between groups;NO content test results showed that the highest is intermittent group,followed by continuous group and control group,and the lowest is the control group,no significant difference(P> 0.05)among the three groups of rats.Conclusion: Six weeks of aerobic treadmill exercise improves spatial learning and memory in rats,in which hippocampus and cerebral cortex of SOD,NO vitality has increased,MDA content has decreased,decreased lipid peroxidation damage in hippocampal and cortical.Compared with the intermittent group and the continuous group,an intermittent exercise to enhance learning and memory ability and free radical scavenging effect is stronger than the continuous exercise.Six weeks of treadmill training to enhance learning and memory may has related to movement enhancing the hippocampus,cortex resistance to free radical damage,so that the body of free radicals produce tolerance and resilience,thereby enhancing the antioxidant capacity and increased expression of NO. |
PDF Full Text Request