Anti-aging Effect And Mechanism Of Camellia Oleifera Oil

Camellia oleifera Abel.is a special oil tree species only founds in China.Because its seed oil contains a large amount of unsaturated fatty acid and tea polyphenol,catechin,squalene and tocopherol,it plays very significant role in antioxidant,bacteriostatic,anti-inflammatory,blood lipid regulation,radiation protection,water retention,skin care and hair growth.It is widely used as high-grade edible oil,medical oil,and increasingly as cosmetic base oil.The purpose of this research is based on D-galactose-induced subacute aging model in mice,through the evaluation of in vitro and in vivo antioxidant capacity and skin homogenate metabolomics test,the effects of Oleifera seed oil on mice skin tissue aging of the aging model has been studied,and to study the anti-aging mechanism of Oleifera seed oil,there to provide a scientific basis for its industrial development and application.This research is mainly divided into following three major parts:1.In this experiment,the fatty acid composition of Oleifera seed oil and Camellia oil was firstly compared by GC-MS.It was found that the fatty acid composition of the two was consistent and among them the oleic acid content was the highest.The total antioxidant capacity of the two was further compared by the method of scavenging DPPH free radical and ABTS free radical.The results showed that the IC₅₀ of the removal of DPPH free radical from Oleifera seed oil was 24.72 g/l,and the IC₅₀ of removing ABTS free radical was 12.03 g/l.The IC₅₀ of the removal of DPPH free radical by Camellia oil was 9.12 g/l,and the IC₅₀ of the ABTS radical was 6.57 g/l.It was found that Camellia oil has stronger ability to scavenge free radicals than Oleifera seed oil does.2.In this experiment,D-galactose subacute mouse skin aging model was established by injecting various amounts of D-galactose and the results suggest that the optimal dose of D-galactose is 1000 mg/?kg·d?.Based on the D-galactose subacute aging model,this experiment used external application of Oleifera seed oil and Camellia oil to measure skin water content,endogenous biochemical indicators and tissue morphology of the mice.The results showed that the skin water content of the Oleifera seed oil group,the Camellia oil group and the VE oil group reached61.44%,65.07%and 63.46%,respectively,which were significantly higher than the water content of the model group of 55.1%.According to the group of Oleifera seed oil,Camellia oil group and VE oil group,the superoxide dismutase?SOD?activity of mouse skin was 54.65±6.72 U/mg prot,59.00±4.27 U/mg prot,63.28±6.85 U/mg prot,respectively,which was significantly higher than model group of 46.87±6.28 U/mg prot.The activity of glutathione peroxidase?GSH-PX?was 64.93±6.82 nmol/mg prot,72.04±7.39 nmol/mg prot,and 74.21±5.00 nmol/mg prot,respectively,which was significantly higher than that of the model group of 44.50±4.56nmol/mg prot.The activities of catalase?CAT?were 3.06±0.58 U/mg prot,3.30±0.20 U/mg prot,and 4.04±0.49 U/mg prot,respectively,which were significantly higher than the model group of 2.02±0.36 U/mg prot.The lipid peroxidation product malondialdehyde?MDA?content in the Oleifera seed oil group?4.31±0.31 nmol/mg prot?,the Camellia oil group?3.80±0.41 nmol/mg prot?,and the VE oil group?1.44±0.40 nmol/mg prot?were significantly lower than the model group?6.80±0.76 nmol/mg prot?.The hydroxyproline?Hyp?content of Oleifera seed oil group?3.02±0.41mg/g?,Camellia oil group?3.26±0.41 mg/g?and VE oil group?3.34±0.18mg/g?were all increased compared with the model group?1.99±0.28 mg/g?.In addition,the skin morphology of the mice in the intervention group was improved,the thickness of the epidermis and dermis increased,and the proportion of collagen fibers increased.3.In this experiment,based on the GC-TOF/MS metabolomics method,the mouse skin tissue samples were analyzed and tested,and the metabolomics analysis process from sample collection,platform analysis,data processing to biological information discovery was completed.The results showed that the intervention can regulate the metabolic disorder caused by D-galactose,and the metabolic pathways involved are alanine,aspartic acid and glutamate metabolism,phenylalanine,tyrosine and tryptophan metabolism and linoleic acid metabolism.