Effect Of Bu Sui Sheng Xue Granule On Expression Of PI3K And AKT In Patients With Chronic Aplastic Anemia

Objective:The changes in the expression of PI3K and AKT in the bone marrow mononuclear cells of the bone marrow mononuclear cells(Chronic aplastic anemia,CAA,hereinafter referred to as chronic aplastic)were observed in the PI3K/AKT pathway mediated by the integrin VLA subfamily before and after the use of MMP in the patients with chronic aplastic anemia(anemia,CAA,hereinafter referred to as chronic aplastic anemia).The expression of PI3K,AKT protein and the hematopoiesis of CAA patients were investigated.The relationship between the pathological process of functional failure and the possible mechanism of the function of Bu Sui Sheng Xue granules is studied from the molecular biology level and enzymology angle.Methods:1.According to the diagnostic criteria,32 patients with CA,A treated with Bu Sui Sheng Xue granula were selected as the treatment group.2.The bone marrow of 10 normal people was collected as the normal group.3.The expression of PI3K and AKT protein in bone marrow mononuclear cells before and after treatment was detected by Western blot(Western-blot)and compared with that of the normal group.Results:The expression level of PI3K and AKT protein in CAA patients was lower than that in normal group(P<0.05),After treatment,the expression level of PI3K and AKT was improved,and there was significant difference compared with that before treatment(P<0.05),but there was significant difference compared with the normal group(P<0.05).Conclusion:1.The expression of PI3K and AKT protein in bone marrow mononuclear cells of CAA patients is low,and PI3K/AKT signal transduction pathway may be involved in the pathological process of CAA patients with bone marrow failure.2.Filling medullary hemopoietic particles may adjust the CAA patients bone marrow mononuclear cells PI3K,and AKT protein expression level,to improve the integrin beta 1 mediated injury of Hematopoietic microenvironment,promoting bone marrow Hematopoietic stem cells(Hematopoietic stem cell,HSC,hereinafter referred to as,HSC)directional differentiation,steady,maintain and improve the patients,the purpose of treatment.