Research On The Determining Sensitive Algae In Measuring Algae Infrared Radiation To Respond To Acute Toxicity Of Antibiotics

Antibiotics as a class of environmental contamination, research about its ecotoxicology which had been strengthened in recent years. In order to analysis acute toxicity of antibiotics used algae infrared radiation, it is necessary to determinate sensitive algae used in toxicity analysis.Sensitive algae had four characteristics of big temperature differences, quick response, many poisons, and low dose responding to acute toxicity when they were used as test materials in algae infrared radiation. Whether sensitive algae exist in natural algae, how to determine sensitive algae was the key of establishing method of algae infrared radiation. Algae have photophosphorylation and oxidative phosphorylation ,two kinds of energy metabolisms systems,when toxic substance invaded,energy emitted in the form of infrared radiation variation could be detected by infrared thermometer. According to this principle, the paper conducted study on the determination of sensitive algae which used in algae infrared radiation detecting antibiotics.Eight species of algae (Selenastrum capricornutum, Anabaena flos-aquae,Oscillatoria agardhii Gom , Fragilaria vaucheriae (Kütz.) Petersen , Nitzschia hantzschia amphioxys(Her.)Grun,Chlamydomonas reinhardtii,Chlorella pyrenoidosa Chick,Scenedesmus obliquus)and ten kinds of antibiotics (Benzylpenicillin,Azithromycin,Cefoperazone,Ceftezole,Ceftriaxone,Mezlocillin,Gatifloxacin,Piperacillin,Amoxicillin,Clindamycin)were used to test in this paper. Portable infrared thermometer was used to test the response of algae to antibiotics. Sensitive algae was initially confirmed by analyzing the test results. Then, it was finally confirmed according to sensitive algae's sensitivity test.Mainly obtained following conclusion:Firstly, antibiotics can cause algae infrared radiation changes which can be detected by portable infrared thermometer.Secondly, there were obvious differerce between different algae.Eight species of algae responded to ten kinds of antibiotics. the average absolute temperature difference was between 0.15℃~0.34℃, the response time was between 3.6min ~ 5.9min, and the percent of responding was between 20%~70%.Thirdly, Chlamydomonas reinhardtii responding to antibiotics ,the average of absolute temperature differences was 0.34℃, the average response time was 5.6min, and the percent of the better responding to antibiotics was 70%. According to first three teams of definition of sensitive algae, Chlamydomonas reinhardtii was initially confirmed as sensitive algae.Fourthly,sensitivity test of Chlamydomonas reinhardtii. the minimal response dose of Chlamydomonas reinhardtii was between 0.00001mg/L~0.1mg/L, and it can attain sensitivity of luminescent bacteria to antibiotics 0.05mg/L~2.0mg/L.。According to complete definition of sensitive algae, Chlamydomonas reinhardtii was finally confirmed as sensitive algaeFifthly, compared with Algae Infrared Radiation used to detecting Heavy Metals, organic toxicities and pesticide ,Algae Infrared Radiation used to detecting antibiotics had relatively long response time, big response tempeture difference, low percent of the responding to antibiotics, and low minimal response dose.