Effects Of Shu Guan Drop Pill On Regulation Of Inflammatory Mediators In Rabbits With Atherosclerosis And Treatment Of Patients With Unstable Angina Pectoris

Objective To observe the treatment effects of Shu Guan Drop Pill on patients with unstable angina pectoris and to explore the possible pharmacological mechanism.Methods 1.The rabbit aoritic atherosclerotic model was developed by high cholesterol feeding. They were randomly divided into three groups: model group(n=6),Shu Guan Drop Pill group(n=7) and Simvastatin group(n=7). At the same time, there were 8 rabbits determined as normal group. The concentrations of plasma sCD40L,soluble intercellular adhesion molecule-l(sICAM-l), and soluble vascular cell adhesion molecule-l(sVCAM-l) were studied by means of Enzyme-linked Immunosorbent Assay (EIA).The immunohistochemistry analysis was used to measure the expression of ICAM-1 in rabbit aortic atherosclerotic plaques. Then the aortas and hearts were harvested for histomorphometry observation.2.Thirty patients with unstable angina pectoris were treated by Shu Guan Drop Pill,in company to thirty patients treated with She Xiang Bao Xin Pill.Results l.The animal experiment study showed that: The levels of serum TC, TG, LDL-C were significantly lower in Shu Guan Drop Pill group than that in model group ( P<0.05 ) ,but the level of serum HDL-C was detected no significant difference between them.The levels of plasmasCD40L, sICAM-1 in Shu Guan Drop Pill group were significantly lower than that in model group ,but no significant difference was detected between them for sVCAM-l.The number of aortic atherosclerotic plaques was significantly less in the Shu Guan Drop Pill group than that in the model group ( P<0.05 ) . However,the number of coronary atherosclerotic plaques did not alter significantly among them.In addition, the ratio of lesion to intimal area,the intima thickness, the thickness ratio of intima to media , and the area ratio of intima to media were also significantly less in the Shu Guan Drop Pill group than that in the model group ( P<0.01 ).2.The clinical study showed that:The total effective rate of Shu Guan Drop Pill on improving clinical symptoms of patients with unstable angina pectoris was 90.00%, and very superior to that of She Xiang Bao Xin Pill ( P<0.05 ). Shu Guan Drop Pill could markedly alleviate pain of patients with unstable angina pectoris. The total effective rate was 86.67%, showing significantly higher advantage over She Xiang Bao Xin Pill ( P<0.05 ) . Shu Guan Drop Pill could improve electrocardiograph(ECG) of patients with myocardial ischemia. Shu Guan Drop Pill could significantly decrease the dosage of nitroglycerin of patients with unstable angina pectoris .The discontinue rate of nitroglycerin was 86.67%, showing very higher advantage over She Xiang Bao Xin Pill ( P<0.05 ). Shu Guan Drop Pill could regulate the abnormality of blood lipid and possessed the actions on declining the level of LDL which induced coronary atherosclerosis and elevating the level of HDL which prevented coronary atherosclerosis, showing significant superiority to She Xiang Bao Xin Pill ( P<0.01 ) .Shu Guan Drop Pill could decrease the levels of plasma sCD40LsVCAM-IsICAM-l,which were significant difference between before and after treatment ( P<0.01 ) .Conclusions These results indicate that Shu Guan Drop Pill treatment can decelerate the progression of atherosclerosis and stabilize or reduce atherosclerotic plaques by regulating the abnormality of blood lipid,by decreasing the levels of plasma sCD40L, sICAM-1 , and by inhibiting expression of ICAM-1 in atherosclerotic plaque. The mechanism of decelerating atherosclerotic progression by Shu Guan Drop Pill may be related to inhibiting inflammatory response in atherosclerotic plaque.