| Dryopteris fragrans (L. DF) is a plant pertaining to Dryopteris genus of Dryopteris family, mainly growing in Wudalianchi area of Heilongjiang Province and north China regions. Up to now, it has not been collected by any pharmacopoeia as a kind of herbal medicine, however it is used widely among the people as a medicine to cure skin diseases for a long time. To develop it into a new medicine to cure dermatosis, a pharmacognostic study of Dryopteris fragrans is made as a key point in the present research work and a partial pharmacodynamics and pharmacodynamic substance basic chemical study is also made. A new medicine Fu-Yang-Ning ointment is developed by use of Dryopteris fragrans as the main ingredient.In this paper, an exploratory study of inhibition effect of DF to epiphyte and bacteria is first made. The experimental results show that: DF enjoys a comparatively strong inhibition effect to either epiphyte or bacteria, especially the inhibition effect to epiphyte is superior or equivalent to Da-Ke-Ning (a kind of medicine) and the extract by use of ethanol is identified as the active principle to sterilize the bacteria. The model of scale on tails of mice is used to observe the effect of Fu-Yang-Ning ointment on model of mice suffering from psoriasis, the experimental results show that: DF has a certain curative effect to psoriasis. An animal model of ringworm on body of a rat is also established to observe the curative effect of DF in different concentrations to the model of ringworm on body of a rat under experiment. The experimental results show that: negative rate of focal skins of the rats in the groups giving a low, medium and high dosage of DF ointment exhibits an ascending tendency after an adverse culture and the focal skins of rats between the group giving a high dosage (2.0g/ml) and the matrical groupafter an adverse culture display an extraordinarily obvious difference(p<0.01), which shows that a high dosage of DF ointment has really a curative effect to the ringworm on body of a rat under experiment resulted from gypsum-shaped Trichophyton. Afterwards, a pharmacognostic study of Dryopteris fragrans is made to reveal its morphological and microscopic characteristics, which is regarded as the necessary work for Dryopteris fragrans to be collected by any pharmacopoeia. Research work in chemistry: a gas phase and mass spectrum combination analysis of CO2 supercritical extract is made, 19 ingredients are identified; by use of reciprocal silica gel and polyamide columnar layer separation etc. methods, 10 compounds are extracted and separated as the main effective chemical ingredients; by making use of physiochemical properties and NMR and MS etc. techniques, the structures of 6 compounds are identified and after a reference is made to the reports in various literatures, 3 phloroglucinol derivants are found respectively as Aspidin PB, Dryofragin and Aspidinol , Aspidinol etc., 4 terpene-like compounds are found as Albicanol, Albicany acetate, a-Cadinene and 10- hydroxyl-15-oxo- a -cadinol etc., and including 2 vegetable steroid compounds as , among which 2 compounds are found for the first time in this plant.Finally, by using DF as the main ingredient, Fu-Yang-Ning ointment is developed, the preparation process is determined by use of the cross test method, the standard for quality of its preparation is specified and its stability test is made. It is scheduled to develop it into a new medicine for use in clinic.Based on Dryopteris fragrans being used as a medicine among the people for a long time and obvious effect to contain the bacteria and combining pharmacognostics, Chinese traditional herbal medicine chemistry and pharmacology with study on preparations...
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