| Dryopteris fragrans (L.) schott, a deciduous perennial herb in Dryopteris family ofDryopteris genus, grows in sloping land with stones of high cold area and forest, or inthe cracks of magma near the volcano, distributes in Heilongjiang, Liaoning, Jilin andHebei Province. In Heilongjiang, it chiefly distributes in Wu-da-lian-chi, Bai-ka-shoumountain of Ta-he.town, alpine region of Da-bai Mountain and the north area ofXiao-xing-an-ling Mountain. Dryopteris fragrans (L.) schott, with strong biologicalactivity, have widely been used to treat psoriasis and rheumatoid arthritis.First, the analgesic effective parts of Dryopteris fragrans (L.) schott were determined.After absorbed in macroporous absorption resin AB-8, water extracts of dryopterisfragrans (L.) schott were eluted with water, 50% ethanol and 95% ethanol. Then theeluates were screened and studied by means of hot-plate test and acetic acid-inducedabdominal constriction test in mice. Statistical analysis results indicated that 50%alcohol eluate has the good analgesic effect (spss 10.0).On the basis of effective parts determined, 9 compounds from 50% ethanol eluatewere isolated by silica gel, ODS, Sephadex LH-20 and preparative HPLC. Among them,chemical structures of 6 compounds were fully determined by 1H-NMR, 13C-NMR,HMBC and DEPT combining ESI-MS. Furthermore it is confirmed thatⅠandⅡare newcompounds. And the other 4 chemicals were discovered first time in the plant ofdryopteris genus. In addition, it was reported that compoundⅢhas effect on preventingand treating diabetes, obesity and cancer.Current medical research indicates that many human diseases are related with freeradicals in body, such as cancer, diabetes and hypertension. This paper studied the freeradical DPPH scavenging capacity of four lignanoids from Dryopteris fragrans (L.) schott by colorimetric method. The results showed that the CompoundⅣandⅥhavegood antioxidant activity with scavenging capacity of 30.24%,24.0% at 50μg/mL. |
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