| Aim:In this study, perform selective neurotomy of several parts of rats'central nervous and peripheral nervous, and use the methods of tectology, biochemistry, and molecular biology, to observe the effect of pre-moxibustionat acupoint Zusanli(ST36) on acute gastric mucosal lesion. And endogenous EGF, SS, IL-2, and HSP-70were checked to explore the mechanism of pre-moxibustion at acupoint Zusanli(ST36) to defense gastric mucosal lesion, if it is ralated to neuroregulation or humoral coordination.Methods:SD rats were randomly divided into7groups:A(Control group), B(Model group), C(Pre-moxibustion group), D(Group of pre-moxibustion+transection of spinal cord), E(Group of pre-moxibustion+damage nucleus of solitary tract) and F(Group of pre-moxibustion+vagotomy), G(Group of pre-moxibustion+neurotomy of common peroneal nerve). Neurotomy of several parts of rats'central nervous and peripheral nervous were performed beforehand, and then pre-moxibustionat acupoint Zusanli(ST36) were carried out. After that acute gastric mucosal lesion models were made. At last, the changes of rats'gastric mucosa were observed under light microscope; UI index were counted by GUTH method; EGF,SS in gastric mucos andIL-2in serum were checked by ELISA method; HSP-70were checked by western-blot method in gastric mucos, ELISA method in serum.Results:1.After modeled, the UI of gastric mucosa of the model group was the highest and the lowest was the normal group. The great significantly difference between these two groups illustrated that model was successful. And in group of pre-moxibustion the gastric mucosa of rats, which was damaged, recovered in various degree (P<0.01), that appeared by the decreased UI, upturn of slough off epidermal cell, and tend to be normal, and so on.2. The effect of pre-moxibustion at acupoint Zusanli(ST36) to defense gastric mucosal lesion in groups of neurotomy ws not that good than that in group of pre-moxibustion(P<0.01, P<0.05). There are some damage on gastric mucosa surface, presence of inflammatory cells, and hyperemia in cells.3. Pre-moxibustion at acupoint Zusanli(ST36) can up-raise the contents of EGF in gastric mucosa,IL-2in serum, and reduce that of SS in gastric mucosa. But this effect were interfered by neurotomy(P<0.01, P<0.05).4. Considerable damage appeared in gastric mucosa of model group; UI indexs, but the expression of HSP-70in serum were of group C, D, E, F and G were considerabley increased vs groupB(P<0.01, P<0.05). And the expression of HSP-70in serum operation groups had no difference with group C(P>0.05).5. The the expression of HSP-70in gastric mucosa of group C, D, E, F, G were higher than group B(P<0.01, P<0.05), but that of operation groups were lower than group C, and the expression of HSP-70in gastric mucosa in group B and group A had no difference(P>0.05).6. There are no difference of the expression of HSP-70in different neurotomy of different parts of nerve tract(nervus peroneus communis, spinal cord, nucleus of solitary tract and vagus)(P>0.05).Conclusion:1.Pre-moxibustion at acupoint Zusanli(ST36) can improve the histomorphology and depress the UI of gastric mucosa of model rats.2.Neurotomy of different parts has obvious interfere in the depress effect of Pre-moxibustion at acupoint Zusanli(ST36) on the UI of gastric mucosa. 3.Neurotomy of different parts has obvious interfere in the effect of Pre-moxibustion at acupoint Zusanli(ST36) on the up-raise the contents of EGF in gastric mucosa, IL-2in serum, and reduce that of SS in gastric mucosa, proving that neuroregulation is important to the mechanism of pre-moxibustion.4.defense effect of pre-moxibustion at acupoint Zusanli(ST36)on gastric mucosal lesion is related to its inducement to expression of HSP-70. And neuroregulation participate the effective channel of signal transduction.5.The nervus peroneus communis, spinal cord, nucleus of solitary tract and vagus are all parts of effective signal passage of pre-moxibustion at acupoint Zusanli(ST36) to gastric mucosal lesion.
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