Study On The Extraction, Isolation And Antioxidant Activity Of The Flavonoid Compounds From The Leaves Of Acanthopanax

Acanthopanax senticosus (Rupr et Maxim.) Harms is Araliaceae and a traditional Chinese medicine, with anti-fatigue, anti-inflammatory, antibacterial, antitumor, cure impotence and so on. Saponins, flavonoids, polysaccharides, trace elements and amino acids etc have been found in Acanthopanax senticosus (Rupr et Maxim.) Harms. This will be the leaves of Acanthopanax senticosus as the research object extraction, separation and antioxidant research.⑴The experimental results show that the ultrasonic extraction method is the best extraction technology by compared with the reflux method and accelerated solvent extraction , the optimal technology are as follows: the ethanol concentration is 20 times of 50%, ethanol extraction time 2 times and 1.5 hours each. The extraction rate is 5.192% . The recovery is 99.98%.⑵HSCCC to isolate the active chemical compounds from the leaves of Acanthopanax. The detection wavelength is 254 nm, the solvent system is Ethyl acetate: Methanol:water(1:0.15:1, V/V/V). With High-speed countercurrent chromatogaphy (HSCCC) isolated from the ethyl acetate layer have rutin and its isomers, chlorogenic acid, quercetin, hyperin and its isomers. The purity of chlorogenic acid is 88.29%. The purity of quercetin is 78.59%. High performance liquid chromatography with electrospray ionization mass spectrometry senticosus extracts on the analysis, combined with the high performance liquid chromatogram and the peak time in the molecular weight, with reference to the literature to determine the crude extract contains rutin and its isomers, hyperin and isomers, two dicaffeoylquinic acids, chlorogenic acid, quercetin glycosides and quercetin have discovered compounds.⑶The antioxidant activity of total flavonoids extract against DPPH and hydroxyl radical has been evaluated. The removal capacity of the extract on DPPH is best. With the concentration increasing and time extending, the removal capacity is increasing. After 30 min, the removal capacity is increasing slowly, and then the IC50 is 0.0865mg/ml. The removal capacity of the extract on DPPH is best, too. With the concentration increasing and time extending, the removal capacity is increasing. There is a certain dose-effect relationship between the removal capacity and the concentration of flavonoid.