Study On Ecological Condition And Nutrient Constituent Of Cardamine Hupingshanensis

Cardamine hupingshanensis, a perennial plant, was a rare wild vegetable which distributed in the narrow space, and being in severe danger of annihilation. To provide science evidence and theory support for exploiting and utilizing the wild vegetable resource, the morphology characters, distribution area as well as life species were investigated, and original life environment, reproduce nature as well as life history were researched, and chemical components were analyzed. On the methods, the determination and separation of water- soluble vitamins in vegetable by reversed phase high performance liquid chromatography was reported. The main results were showed as follow:1 Morphology characters of HUPCardamine hupingshanensis is up to 30⁶0cm tall. The leaves have two shapes and the plant has terminal raceme. The white flower has 4 oblong sepals and 4 sub-circular petal. The flower has 6 stamens with some flat filaments, and the stamens are tetradynamous. The tow outer stamens are shorter and the four inner stamens are longer. Between the base of filaments there are one deep green nectary with scale form respectively. Ovary has the form of slight cylinder. Style is shorter and stigma is head shape with the form of hemisphere. The fruit is silique, and it is some flat with the shape of slight cylinder. There are 6¹2 brownish seeds with the form of sub-roundness and broad-rectangle. The surface of seed is relative smoothness.2 Ecological condition of HUPCardamine hupingshanensis, found only in the huping mountain in hunan shimen country, were distributed in the environment with an elevation from 800 to 1400m and rainfall 1800 to 2100mm. In the distributing area, the soil was black and thickness of humus was from 0.5to 1.0cm. The annual average temperature was 15.4℃,even in the august, extreme temperature only up to 30.6℃. HUP always lived in the mountain stream and grow well under the lowest forest. In the noon, when the illumination intensity was strong, the photosynthetic rate of HUP became slower.3 Reproduce nature of HUPHUP multiplied mainly by alimentative reproduction. The reproduction ability was so strong that the buds in the basal stem and the root could grow up as well as those in the stem and the branches. In April pre year, the alimentation growth turned to sexual growth, and it bourgeoned in early May and blossomed in later stage. The seeds were so small as fruits matured in later June that it could not germinate in the nature. During the period from July to August, branches began to lodge and came into the alimentative growth, which continued to the next March. The life history of plants presented as perennial.4 Analysis of chemical components in HUPAnalysis of nutrient constituents showed that Cardamine hupingshanensis posses high contents in vitamin C, BI, B2 and PP. Especial, the contents of vitaminBi was as 9 times as that of Chinese cabbage that was rarely discovered in normal wild vegetable. It also owned high mineral elements such as Ca, Mg, Mn, Zn, otherwise many essential minim elements. Furthermore, the essential amino acids was completely detected, and the content of three limited amino acids were as 10.3% as that of total amino acids.5 Analysis of water- soluble vitamins in vegetable with HPLCA method for determination of water-soluble vitamin C, B₁ B₂ and nicotinic acid in Cardamine hupingshanensis by reversed phase high performance liquid chromatography was reported. The four kinds of vitamins were separated on uBondparkCis column using a buffer solution of methanol-water (with O.lmol/L NaAc-HAc, 0.02% triethylamine, pH3.98) as the mobile phase at a velocity of Iml/min and detected by ultraviolet spectrum-photometric detector at 254nm. The recoveries were 107.7% -96.1% and RSD was less than 3.90%, and correlation coefficient was more than 0.9950. The extracts of Cardamine hupingshanensis by HPO₃ at 70℃ could be measured directly.6 Tissue culture of HUPCallus was induced from the explants including stem nod, leaves, hypocot...