Development And Study On New Drug Of Rifampin Combined Medicament

RFP was dissolved by proper solvent, then select bacteroidal disease's bacteria which were famillar in clinic, at the same time, we tested sesentive activity of bacteria for RFP through drug-sensitivity test,according as this results we educed the antimicrobial activity chart of RFP. To study and compare the antimicrobial activity of Rifampin Combined Medicament in vitro.The MIC determinations of single drug, six combined drugs and Rifampin Combined Medicament, against 5 isolates of veterinary origin were performed using the method of both two-fold dilutions and liquid-chessboard diluted method.The results showed that , Ciprofloxacin, Gentamycin, Neomycin,Enoxacin,Amikacin were more active than other drugs in this examination. Furthermore, if they were combined with RFP ,the MICs were all decreased. After appended adjuvant to Rifampin+Amikacin, the anti-microbial activity was improved remarkably. Conclusion: Rifampin Combined Medicament has good anti-microbial activity in vitro models. After cooperated sterilize drugs were added to Rifampin Combined Medicament,we carried on the anti-Rafimpin bacteria control test and through logical combination, we developed new combined medicament which was composed by Chinese traditional medicine and Weston medicine and its primary ingredient were Rifampin,effective distilled substance of Chinese traditional medicine, bacterial's DNA enzyme inhibit factor, target inducing factor, metabolized inducing factor, etc. At the same time, we develop the drug's powdery shape and tested the stability under some kinds of ordinary conditions to find the optimal preserve condition.In order to offer sufficient scientific basis for the usage of Rifampin Combined Medicament, this study through animal test analysed the metabolism of Rifampin Combined Medicament in goat's blood and milk, then detected bovine mastitis in a cattle farm. At the same time, the therapeutic study with Rifampin Combined Medicament was carried out in bovine clinical mastitis, abserved the metabolizing in its body ,the results showed that medicine in the breast can keep a long time with effective concentration and make the inflammation disappeared in a period of treatment(three-five days).Point injection has the similar effect, but difficult to handle in clinic; basal injection also has well effect in local circle; if we use muscle injection, medicine only can keep a short time .Through the experiment, we can deduce that Rifampin Combined Medicament has good anti-microbial activity in vitro models, also, it can produce very good treated effect for some diseases which was difficult to cure for medicine not easy to arrive the focus such as mastitis. Besides above merit, Rifampin Combined Medicament can reduce the anti-Rafimpin bacteria come into being and enhance the treated effect.